Dührsen U, Metcalf D, Spangrude G J, Nicola N A
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Leukemia. 1989 Nov;3(11):796-803.
PGM-1 is a transplantable leukemia of C3H/HeJ mice growing as a population of undifferentiated blast cells with a predisposition to form subcutaneous tumors and to grow in lymphoid organs. Cell survival and proliferation in vitro are absolutely dependent on stimulation by hemopoietic growth factors, and up to 100% of tumor cells can form colonies of mature granulocytes and/or macrophages in semisolid cultures, the colonies containing no clonogenic cells. Most clonogenic cells in the leukemic population respond to stimulation by multi-colony-stimulating factor (IL-3) or GM-CSF, but some respond also to M-CSF, G-CSF, IL-4, IL-5, or IL-6. In their surface phenotype and proliferative characteristics in vitro, PGM-1 leukemic cells resemble normal granulocyte-macrophage progenitor cells, and the leukemia may be a useful model for human chronic myeloid leukemia.
PGM-1是C3H/HeJ小鼠的一种可移植性白血病，由一群未分化的原始细胞构成，易于形成皮下肿瘤并在淋巴器官中生长。体外细胞存活和增殖绝对依赖于造血生长因子的刺激，高达100%的肿瘤细胞可在半固体培养物中形成成熟粒细胞和/或巨噬细胞集落，这些集落不含克隆形成细胞。白血病群体中的大多数克隆形成细胞对多集落刺激因子（IL-3）或粒细胞-巨噬细胞集落刺激因子（GM-CSF）的刺激有反应，但有些细胞也对巨噬细胞集落刺激因子（M-CSF）、粒细胞集落刺激因子（G-CSF）、白细胞介素-4（IL-4）、白细胞介素-5（IL-5）或白细胞介素-6（IL-6）有反应。PGM-1白血病细胞在其表面表型和体外增殖特性方面类似于正常粒细胞-巨噬细胞祖细胞，这种白血病可能是人类慢性髓性白血病的有用模型。
