Dogan Aysun Sanal, Onder Evrim, Arikok Ata Turker, Bicer Tolga, Gurdal Canan
a Department of Ophthalmology , Ministry of Health and.
b Department of Pathology , Ministry of Health, Diskapi Yildirim Beyazit Training and Research Hospital , Ankara , Turkey.
Cutan Ocul Toxicol. 2016 Dec;35(4):315-8. doi: 10.3109/15569527.2015.1124887. Epub 2016 Jan 28.
Pterygium is the fibrovascular growth of the limbal conjunctiva over cornea. This proliferative nature might have a pathogenesis associated with tight junction proteins.
To investigate the tight junction protein claudin-1 expressions in pterygium with respect to normal conjunctiva.
This retrospective study included 28 patients who underwent pterygium surgery with autograft. Claudin-1 expressions were immunohistochemically evaluated in normal and lesional conjunctiva of the same eye. Immunohistochemical evaluation was done with regard of both the intensity and the extent of staining. The distribution of the immunohistochemical scores in pterygium and normal conjunctiva has been compared with using McNemar test.
The mean age of the patients was 52.2 ± 11.2 years and male/female ratio was 8/20. Among 28 samples of normal conjunctiva 25 (89.2%) demonstrated a strong immunohistochemical expression with claudin-1 whereas this rate was 10.8% for pterygium samples. Statistical analysis revealed a significant decrease in claudin-1 expressions in pterygium with respect to normal conjunctiva (p < 0.001).
The loss of claudin-1 appears to be involved in the pathogenesis of pterygium and the future studies will elucidate the exact role of tight junction proteins in the invasive and recurrent nature of pterygium.
翼状胬肉是角膜缘结膜的纤维血管性生长。这种增殖特性可能与紧密连接蛋白有关的发病机制。
研究紧密连接蛋白claudin-1在翼状胬肉与正常结膜中的表达情况。
这项回顾性研究纳入了28例行自体移植翼状胬肉手术的患者。对同一只眼睛的正常结膜和病变结膜进行claudin-1表达的免疫组织化学评估。免疫组织化学评估从染色强度和范围两方面进行。采用McNemar检验比较翼状胬肉和正常结膜中免疫组织化学评分的分布情况。
患者的平均年龄为52.2±11.2岁，男女比例为8/20。在28份正常结膜样本中，25份（89.2%）显示claudin-1有强免疫组织化学表达，而翼状胬肉样本的这一比例为10.8%。统计分析显示，与正常结膜相比，翼状胬肉中claudin-1表达显著降低（p<0.001）。
claudin-1的缺失似乎参与了翼状胬肉的发病机制，未来的研究将阐明紧密连接蛋白在翼状胬肉侵袭性和复发性中的具体作用。
