绿液化学物质的再利用预处理全稻废物生物质及其在 2,3-丁二醇生产中的应用。
Reutilization of green liquor chemicals for pretreatment of whole rice waste biomass and its application to 2,3-butanediol production.
机构信息
Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul 136-713, South Korea.
Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul 136-713, South Korea.
出版信息
Bioresour Technol. 2016 Apr;205:90-6. doi: 10.1016/j.biortech.2016.01.028. Epub 2016 Jan 20.
The performance of green liquor pretreatment using Na2CO3 and Na2SO3 and its optimization for whole rice waste biomass (RWB) was investigated. Incubation of Na2CO3-Na2SO3 at a 1:1 ratio (chemical charge 10%) for 12% RWB at 100°C for 6h resulted in maximum delignification (58.2%) with significant glucan yield (88%) and total sugar recovery (545mg/g of RWB) after enzymatic hydrolysis. Recovery and reusability of the resulting chemical spent wash were evaluated to treat RWB along with its compatibility for enzymatic digestibility. Significant hydrolysis and lignin removal were observed for up to three cycles; however, further reuse of Na2CO3 and Na2SO3 lowered their performance. Significant 2,3-butanediol (BDO) was produced by Klebsiella pneumoniae KMK-05 with the RWB enzymatic hydrolysate from each pretreatment cycle. BDO yield achieved using RWB-derived sugars was similar to those using laboratory-grade sugars. This pretreatment strategy constitutes an ecofriendly, cost-effective, and practical method for utilizing lignocellulosic biomass.
研究了使用 Na2CO3 和 Na2SO3 进行绿液预处理的性能及其对全稻秸秆生物质（RWB）的优化。在 100°C 下，12%的 RWB 用 1:1 比例（化学计量 10%）的 Na2CO3-Na2SO3 孵育 6 小时，可实现最大脱木质素（58.2%），酶解后有显著的葡聚糖产率（88%）和总糖回收率（545mg/g RWB）。评估了所得化学废碱液的回收和再利用，以处理 RWB，以及其对酶可消化性的兼容性。在三个循环内观察到显著的水解和木质素去除；然而，进一步重复使用 Na2CO3 和 Na2SO3 会降低其性能。利用各预处理循环的 RWB 酶解产物，肺炎克雷伯氏菌 KMK-05 可大量生产 2,3-丁二醇（BDO）。使用 RWB 衍生糖获得的 BDO 产率与使用实验室级糖获得的产率相似。该预处理策略构成了一种环保、经济且实用的利用木质纤维素生物质的方法。
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