Shubin F N, Gintsburg A L, Kitaev V M, Ianishevskiĭ N V, Zenkova Z G
Mol Gen Mikrobiol Virusol. 1989 Jun(6):20-5.
Electrophoresis in agarose gel has been used to study the plasmid spectra of 854 Yersinia pseudotuberculosis strains isolated from different sources. The plasmids found in the microbial strains are represented by the elements with molecular masses 82; 57; 45; 5.5; 4.4; 3.5; 2.7; 2.4; 2.3 Md. The variable spectra of plasmids is peculiar only for serovar I of Yersinia pseudotuberculosis. Plasmids p45 and p82 are classified as the main, while other plasmids as auxiliary ones. In accord with the classification all plasmid containing strains are divided into 8 plasmid strains. Using the proposed method for intraspecific typing of Yersinia pseudotuberculosis permits one to perfect the epidemiological analysis of pseudotuberculosis infection and make concrete the direction of prophylactic and antiepidemic measures.
采用琼脂糖凝胶电泳法研究了从不同来源分离出的854株假结核耶尔森氏菌的质粒图谱。在这些微生物菌株中发现的质粒由分子量为82、57、45、5.5、4.4、3.5、2.7、2.4、2.3 Md的元件代表。质粒的可变图谱仅在假结核耶尔森氏菌血清型I中独特。质粒p45和p82被归类为主质粒,而其他质粒为辅助质粒。根据该分类,所有含质粒菌株被分为8个质粒菌株。使用所提出的假结核耶尔森氏菌种内分型方法可以完善对假结核感染的流行病学分析,并明确预防和抗疫措施的方向。