Demir Melike, Taylan Mahsuk, Kaya Halide, Ekinci Aysun, Arslan Demet, Aslan Emel, Keles Aysenur, Yılmaz Sureyya, Sezgi Cengizhan
a Department of Chest Disease , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
b Department of Biochemistry , Dicle University Faculty of Medicine , Diyarbakir , Turkey.
J Invest Surg. 2016 Oct;29(5):302-8. doi: 10.3109/08941939.2015.1111474. Epub 2016 Jan 29.
The aim of this study was to investigate the role of Ecballium elaterium (EE) on sepsis-induced lung injury.
A total of 30 male rats were divided into three groups as follows: control, sepsis, and treatment groups (sepsis + EE) with each group containing 10 rats. A rat model of sepsis induced by cecal ligation and puncture (CLP) was used. In the treatment group, rats were injected intraperitoneally with 2.5 mg/kg EE after CLP. Interleukin-6 (IL-6), tumor necrosis factor (TNF)-α, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values after a 24-hr period were measured via cardiac puncture. Animals were harvested after the procedure and biochemical analysis was done and histopathological changes of the tissue sections of lungs were examined thereafter.
A statistically significant decrease was observed in the IL-6 (p < .05), TNF-α (p < .05), and TOS (p < .01) levels in the sera of the treatment group compared to those of the sepsis group. Following the treatment, the TOS (p = .01) and OSI (p < .05) levels in the lung tissue of rats indicated a statistically significant decrease compared to those of the sepsis group. The histopathological follow-up undertaken after the administration of the EE treatment to septic rats showed significantly lower values of alveolar wall thickness (p < .001), interstitial edema (p = .018), and neutrophil infiltration (p = .047).
EE treatment may have beneficial effects on sepsis-induced lung injury, and therefore has potential for clinical use.
本研究旨在探讨喷瓜（EE）在脓毒症诱导的肺损伤中的作用。
总共30只雄性大鼠被分为以下三组：对照组、脓毒症组和治疗组（脓毒症+EE），每组10只大鼠。采用盲肠结扎穿孔（CLP）诱导的大鼠脓毒症模型。在治疗组中，大鼠在CLP后腹腔注射2.5mg/kg EE。24小时后通过心脏穿刺测量白细胞介素-6（IL-6）、肿瘤坏死因子（TNF）-α、总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI）值。手术后处死动物并进行生化分析，随后检查肺组织切片的组织病理学变化。
与脓毒症组相比，治疗组血清中的IL-6（p<.05）、TNF-α（p<.05）和TOS（p<.01）水平有统计学意义的降低。治疗后，大鼠肺组织中的TOS（p=.01）和OSI（p<.05）水平与脓毒症组相比有统计学意义的降低。对脓毒症大鼠进行EE治疗后的组织病理学随访显示，肺泡壁厚度（p<.001）、间质水肿（p=.018）和中性粒细胞浸润（p=.047）的值显著降低。
EE治疗可能对脓毒症诱导的肺损伤有有益作用，因此具有临床应用潜力。
