Arslan Mehmet Serif, Basuguy Erol, Ibiloglu Ibrahim, Bozdemir Eda, Zeytun Hikmet, Sahin Atalay, Kaplan Ibrahim, Aydogdu Bahattin, Otcu Selcuk
a Department of Pediatric Surgery , University of Dicle , Diyarbakir , Turkey.
b Department of Pathology , University of Dicle , Diyarbakir , Turkey.
J Invest Surg. 2016 Dec;29(6):399-404. doi: 10.1080/08941939.2016.1181230. Epub 2016 May 18.
Ecballium elaterium (EE) is a plant from Cucurbitaceae family. Its anti-inflammatory role in sepsis is not well understood. We investigated the effects of EE on serum levels of proinflammatory cytokines and further explored the mechanisms underlying histological changes in liver and ileum following EE administration in a polymicrobial sepsis model.
Thirty rats were divided into three groups of 10 rats each. Rats were subjected to sham laparotomy plus normal saline administration (control group, CG), laparotomy with cecal ligation and puncture (CLP) (sepsis group, SG), and laparotomy with CLP plus 2.5 mg/kg EE administration (experimental group, EG). Twenty-four hours after laparotomy, animals underwent cardiac puncture, and blood was collected for interleukin 1 (IL-1), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) assessment. Whole sections of liver and ileum tissues were collected for histologic examination.
The serum level of IL-6 was significantly lower in EG as compared to SG. Although IL-6 levels were shown a statistically significant (p < 0.0001) decline to near control values, no significant changes were observed in serum levels of IL-1 and TNF-α after EE treatment. Histologic examination revealed statistically significant reduction in collagen formation (p = 0.001) on serosal surface of ileum and hepatic venous congestion (p = 0.040) in EG as compared to SG.
EE might play a protective role in sepsis prevention and treatment by decreasing IL-6 production and reducing liver damage and may influence bacterial translocation by reinforcing intestinal barrier function.
喷瓜是一种来自葫芦科的植物。其在脓毒症中的抗炎作用尚未完全明确。我们研究了喷瓜对促炎细胞因子血清水平的影响，并进一步探讨了在多微生物脓毒症模型中给予喷瓜后肝脏和回肠组织学变化的潜在机制。
将30只大鼠分为三组，每组10只。对大鼠进行假剖腹术并给予生理盐水（对照组，CG）、剖腹术加盲肠结扎和穿刺（CLP）（脓毒症组，SG）以及剖腹术加CLP并给予2.5mg/kg喷瓜（实验组，EG）。剖腹术后24小时，对动物进行心脏穿刺，采集血液用于评估白细胞介素1（IL-1）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）。收集肝脏和回肠组织的全切片用于组织学检查。
与SG组相比，EG组的IL-6血清水平显著降低。虽然IL-6水平显示出统计学上显著（p<0.0001）下降至接近对照值，但喷瓜治疗后IL-1和TNF-α的血清水平未观察到显著变化。组织学检查显示，与SG组相比，EG组回肠浆膜表面的胶原形成（p = 0.001）和肝静脉淤血（p = 0.040）有统计学上的显著减少。
喷瓜可能通过降低IL-6的产生、减轻肝脏损伤在脓毒症的预防和治疗中发挥保护作用，并可能通过增强肠道屏障功能影响细菌移位。
