Funato T, Take A, Ichikawa K, Ohtani H, Kankazi T
Department of Clinical Pathology, Kitasato University of Medicine, Sagamihara, Japan.
Neoplasma. 1989;36(5):513-8.
Samples obtained from six human melanoma cell lines have been tested for the presence of p21 ras oncogene product by Western blotting analysis. The overexpression of p21 was detected in two cell lines out of the six samples. These DNA samples have not been shown to be transformants by transfection assay. Moreover, amplification or rearrangement of DNAs from these cell lines was not found by Southern blotting analysis. Northern blotting analysis, however, detected the expression of Ki-ras gene in RNA extracted from the two cell lines. Thus, it was suggested that the overexpression of p21 might be a result of increased normal mRNA transcript of Ki-ras gene.
通过蛋白质免疫印迹分析，检测了取自六种人黑色素瘤细胞系的样本中p21 ras癌基因产物的存在情况。在六个样本中的两个细胞系中检测到了p21的过表达。通过转染试验未显示这些DNA样本为转化体。此外，通过Southern印迹分析未发现这些细胞系的DNA发生扩增或重排。然而，Northern印迹分析在从这两个细胞系提取的RNA中检测到了Ki-ras基因的表达。因此，有人提出p21的过表达可能是Ki-ras基因正常mRNA转录增加的结果。
