Li Wenting, Zeng Zhiyu, Gui Chun, Zheng Huilei, Huang Weiqiang, Wei Heng, Gong Danping
Department of Cardiology, The First Affiliated Hospital, Guangxi Medical UniversityNanning 530021, Guangxi, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical UniversityNanning, Guangxi 530021, China.
Department of Cardiology, The First Affiliated Hospital, Guangxi Medical University Nanning 530021, Guangxi, China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):14151-60. eCollection 2015.
Rheumatic heart disease (RHD) makes a heavy burden in human lives and economy. The proteomic analysis of acute rheumatic heart disease (ARHD) can provide precious data to study RHD at the early stages, but no one has looked into. So based on our early research we applied the method of continuous GAS stimulation on Lewis rats to duplicate the animal model of ARHD. And the mitral valves of rats in control group (n=10) and ARHD group (n=10) were selected for proteomic analysis of ARHD with the iTRAQ labeling based 2D LC-ESI-MS/MS quantitative technology. We identified 3931 proteins in valve tissue out of which we obtained 395 differentially expressed proteins containing 176 up-regulated proteins and 119 down-regulated proteins. Changes in levels of GAPDH (6.793 times higher than the control group) and CD9 (2.63 times higher than the control group) were confirmed by Western blot or immunohistochemistry. The differentially expressed proteins such as GAPDH, CD9, myosin, collagen and RAC1 may be potential biomarkers for ARHD. Moreover, the mitral valve protein profile shed light on further understanding and investigating ARHD.
风湿性心脏病（RHD）给人类生命和经济带来沉重负担。急性风湿性心脏病（ARHD）的蛋白质组学分析可为早期研究RHD提供宝贵数据，但此前尚无相关研究。因此，基于我们早期的研究，我们采用对Lewis大鼠进行连续A组乙型溶血性链球菌（GAS）刺激的方法来复制ARHD动物模型。选取对照组（n = 10）和ARHD组（n = 10）大鼠的二尖瓣，采用基于iTRAQ标记的二维液相色谱-电喷雾串联质谱定量技术对ARHD进行蛋白质组学分析。我们在瓣膜组织中鉴定出3931种蛋白质，其中获得395种差异表达蛋白质，包括176种上调蛋白质和119种下调蛋白质。通过蛋白质免疫印迹法或免疫组织化学法证实了甘油醛-3-磷酸脱氢酶（GAPDH，比对照组高6.793倍）和CD9（比对照组高2.63倍）水平的变化。GAPDH、CD9、肌球蛋白、胶原蛋白和RAC1等差异表达蛋白质可能是ARHD的潜在生物标志物。此外，二尖瓣蛋白质谱有助于进一步理解和研究ARHD。
