Zheng Dawei, Xu Limin, Sun Lebo, Feng Qiang, Wang Zishan, Shao Guofeng, Ni Yiming
Department of Cardiothoracic Surgery, The Affiliated Hospital, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China ; Department of Cardiothoracic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
Department of Cardiothoracic Surgery, The Affiliated Hospital, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China.
Biomed Res Int. 2014;2014:151726. doi: 10.1155/2014/151726. Epub 2014 Mar 12.
Rheumatic heart disease (RHD) is a serious autoimmune heart disease. The present study was aimed at identifying the differentially expressed proteins between patients with RHD and controls with mitral valve prolapse.
Nine patients with RHD and nine controls with mitral valve prolapsed were enrolled for this study. Two-dimensional difference in-gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were performed.
A total of 39 protein spots with differential expressions were identified between the two groups (P < 0.05, Average Ratio > 1.2 or Average Ratio < -1.2) and four upregulated proteins (including heat shock protein 60 (HSP 60), desmin, PDZ and LIM domain protein 1, and proteasome subunit alpha type-1) and three downregulated proteins (including tropomyosin alpha-1 chain, malate dehydrogenase, and chaperone activity of bc1 complex homolog) were determined.
These seven proteins, especially HSP 60, may serve as potential biomarkers for the diagnosis of RHD and provide evidence to explain the mechanisms of this complex disease in the future.
风湿性心脏病（RHD）是一种严重的自身免疫性心脏病。本研究旨在鉴定RHD患者与二尖瓣脱垂对照组之间差异表达的蛋白质。
本研究纳入了9例RHD患者和9例二尖瓣脱垂对照者。进行了二维差异凝胶电泳（2D-DIGE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析。
两组之间共鉴定出39个差异表达的蛋白质点（P < 0.05，平均比值> 1.2或平均比值< -1.2），并确定了4种上调蛋白（包括热休克蛋白60（HSP 60）、结蛋白、PDZ和LIM结构域蛋白1以及蛋白酶体α亚基-1）和3种下调蛋白（包括原肌球蛋白α-1链、苹果酸脱氢酶和bc1复合体同源物的伴侣活性）。
这7种蛋白质，尤其是HSP 60，可能作为RHD诊断的潜在生物标志物，并为今后解释这种复杂疾病的机制提供证据。
