由MgrB调节因子失活引起的黏菌素耐药性与序列型258产KPC碳青霉烯酶肺炎克雷伯菌的毒力降低无关。
Colistin Resistance Caused by Inactivation of the MgrB Regulator Is Not Associated with Decreased Virulence of Sequence Type 258 KPC Carbapenemase-Producing Klebsiella pneumoniae.
作者信息
Arena Fabio, Henrici De Angelis Lucia, Cannatelli Antonio, Di Pilato Vincenzo, Amorese Marina, D'Andrea Marco Maria, Giani Tommaso, Rossolini Gian Maria
机构信息
Department of Medical Biotechnologies, University of Siena, Siena, Italy.
Department of Surgery and Translational Medicine, University of Florence, Florence, Italy.
出版信息
Antimicrob Agents Chemother. 2016 Mar 25;60(4):2509-12. doi: 10.1128/AAC.02981-15. Print 2016 Apr.
Abstract
Using aGalleria mellonellaanimal model, we compared the virulence of two sequence type 258 (ST258) KPC-producingKlebsiella pneumoniaestrains, which were representative of the two clades of this clonal lineage, with that of isogenic colistin-resistantmgrBmutants. With both strains, themgrBmutants did not exhibit modification in virulence. In theG. mellonellamodel, the clade 1 strain (capsular typecps-1[wzi29, producing KPC-2]) was significantly more virulent than the clade 2 strain (capsular typecps-2[wzi154, producing KPC-3]).
摘要
利用家蚕模型，我们比较了两个序列型258（ST258）产KPC的肺炎克雷伯菌菌株（它们代表了该克隆谱系的两个分支）与同基因耐黏菌素mgrB突变体的毒力。对于这两个菌株，mgrB突变体的毒力均未表现出改变。在家蚕模型中，分支1菌株（荚膜型cps-1[wzi29，产KPC-2]）的毒力显著高于分支2菌株（荚膜型cps-2[wzi154，产KPC-3]）。
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本文引用的文献
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Lancet Infect Dis. 2016 Feb;16(2):161-8. doi: 10.1016/S1473-3099(15)00424-7. Epub 2015 Nov 19.
2
Risk factors for bloodstream infections due to colistin-resistant KPC-producing Klebsiella pneumoniae: results from a multicenter case-control-control study.
Clin Microbiol Infect. 2015 Dec;21(12):1106.e1-8. doi: 10.1016/j.cmi.2015.08.001. Epub 2015 Aug 14.
3
Large Nosocomial Outbreak of Colistin-Resistant, Carbapenemase-Producing Klebsiella pneumoniae Traced to Clonal Expansion of an mgrB Deletion Mutant.
J Clin Microbiol. 2015 Oct;53(10):3341-4. doi: 10.1128/JCM.01017-15. Epub 2015 Jul 22.
4
Heteroresistance to colistin in Klebsiella pneumoniae associated with alterations in the PhoPQ regulatory system.
Antimicrob Agents Chemother. 2015 May;59(5):2780-4. doi: 10.1128/AAC.05055-14. Epub 2015 Mar 2.
5
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Antimicrob Agents Chemother. 2015 May;59(5):2909-13. doi: 10.1128/AAC.04763-14. Epub 2015 Feb 17.
6
Polymyxin resistance caused by mgrB inactivation is not associated with significant biological cost in Klebsiella pneumoniae.
Antimicrob Agents Chemother. 2015 May;59(5):2898-900. doi: 10.1128/AAC.04998-14. Epub 2015 Feb 17.
7
Extensively drug-resistant carbapenemase-producing Enterobacteriaceae: an emerging challenge for clinicians and healthcare systems.
J Intern Med. 2015 May;277(5):528-31. doi: 10.1111/joim.12350. Epub 2015 Feb 16.
8
Colistin resistance superimposed to endemic carbapenem-resistant Klebsiella pneumoniae: a rapidly evolving problem in Italy, November 2013 to April 2014.
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9
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10
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