高产碳青霉烯酶肺炎克雷伯菌中 基因各种突变介导的对黏菌素高水平耐药。
High-Level Resistance to Colistin Mediated by Various Mutations in the Gene among Carbapenemase-Producing Klebsiella pneumoniae.
机构信息
Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Fribourg, Switzerland.
出版信息
Antimicrob Agents Chemother. 2017 Oct 24;61(11). doi: 10.1128/AAC.01423-17. Print 2017 Nov.
Abstract
Mutations in genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.
摘要
在一组耐粘菌素的分离株中，搜索了编码参与脂多糖修饰的双组分调节子的基因突变。与野生型菌株相比，分别产生碳青霉烯酶 NDM-1、OXA-181 或 KPC-2 的 4 株分离株的 CrrB 蛋白发生了突变。用野生型 CrrB 蛋白进行互补试验，在所有情况下都恢复了对粘菌素的敏感性，这证实了所鉴定的取代与耐药表型有关。
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