Rees David J, Hanifi Arash, Manion Joseph, Gantayet Arpita, Sone Eli D
a Institute of Biomaterials & Biomedical Engineering , University of Toronto , Toronto , Canada.
b Department of Materials Science & Engineering , University of Toronto , Toronto , Canada.
Biofouling. 2016;32(2):205-13. doi: 10.1080/08927014.2015.1135426.
The invasive freshwater mollusc Dreissena bugensis (quagga mussel) sticks to underwater surfaces via a proteinacious 'anchor' (byssus), consisting of a series of threads linked to adhesive plaques. This adhesion results in the biofouling of crucial underwater industry infrastructure, yet little is known about the proteins responsible for the adhesion. Here the identification of byssal proteins extracted from freshly secreted byssal material is described. Several new byssal proteins were observed by gel electrophoresis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to characterize proteins in different regions of the byssus, particularly those localized to the adhesive interface. Byssal plaques and threads contain in common a range of low molecular weight proteins, while several proteins with higher mass were observed only in the plaque. At the adhesive interface, a plaque-specific ~8.1 kDa protein had a relative increase in signal intensity compared to the bulk of the plaque, suggesting it may play a direct role in adhesion.
入侵性淡水软体动物波氏褶纹冠蚌（斑马贻贝）通过一种由一系列与粘性斑相连的细丝组成的蛋白质“锚”（足丝）附着在水下表面。这种附着会导致关键水下工业基础设施的生物污染，但对于负责这种附着的蛋白质却知之甚少。本文描述了从新分泌的足丝材料中提取的足丝蛋白的鉴定过程。通过凝胶电泳观察到了几种新的足丝蛋白。基质辅助激光解吸/电离飞行时间质谱用于表征足丝不同区域的蛋白质，特别是那些定位于粘附界面的蛋白质。足丝斑和细丝共同含有一系列低分子量蛋白质，而几种较高分子量的蛋白质仅在斑中观察到。在粘附界面，一种斑特异性的约8.1 kDa蛋白质与斑的主体相比信号强度相对增加，表明它可能在粘附中起直接作用。
