Institute of Biomaterials & Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
Department of Materials Science & Engineering, University of Toronto, Toronto, ON, Canada.
Sci Rep. 2019 Apr 19;9(1):6305. doi: 10.1038/s41598-019-41976-7.
The European freshwater mollusk Dreissena bugensis (quagga mussel), an invasive species to North America, adheres to surfaces underwater via the byssus: a non-living protein 'anchor'. In spite of its importance as a biofouling species, the sequence of the majority of byssal proteins responsible for adhesion are not known, and little genomic data is available. To determine protein sequence information, we utilized next-generation RNA sequencing and de novo assembly to construct a cDNA library of the quagga mussel foot transcriptome, which contains over 200,000 transcripts. Quagga mussel byssal proteins were extracted from freshly induced secretions and analyzed using LC-MS/MS; peptide spectra were matched to the transcriptome to fingerprint the entire protein primary sequences. We present the full sequences of fourteen novel quagga mussel byssal proteins, named Dreissena bugensis foot proteins 4 to 17 (Dbfp4-Dbfp17), and new sequence data for two previously observed byssal proteins Dbfp1 and Dbfp2. Theoretical masses of the newly discovered proteins range from 4.3 kDa to 21.6 kDa. These protein sequences are unique but contain features similar to glue proteins from other species, including a high degree of polymorphism, proteins with repeated peptide motifs, disordered protein structure, and block structures.
欧洲淡水软体动物斑马贻贝（贻贝），一种入侵北美的物种，通过贻贝附着在水下表面：一种无生命的蛋白质“锚”。尽管它作为生物污垢物种非常重要，但负责粘附的大多数贻贝蛋白的序列尚不清楚，并且可用的基因组数据很少。为了确定蛋白质序列信息，我们利用下一代 RNA 测序和从头组装构建了斑马贻贝足部转录组的 cDNA 文库，其中包含超过 200,000 个转录本。从新诱导的分泌物中提取斑马贻贝的贻贝蛋白，并使用 LC-MS/MS 进行分析; 将肽谱与转录组匹配以确定整个蛋白质一级序列。我们介绍了 14 种新型斑马贻贝贻贝蛋白的完整序列，命名为斑马贻贝足部蛋白 4 到 17（Dbfp4-Dbfp17），以及两种先前观察到的贻贝蛋白 Dbfp1 和 Dbfp2 的新序列数据。新发现的蛋白质的理论质量范围为 4.3 kDa 至 21.6 kDa。这些蛋白质序列是独特的，但包含与其他物种的胶蛋白相似的特征，包括高度多态性、具有重复肽基序的蛋白质、无规蛋白质结构和块结构。
