Sullivan W G
Center for Fetal Diagnosis and Therapy, Wayne State University School of Medicine, Detroit, Mich.
Plast Reconstr Surg. 1989 Nov;84(5):723-30; discussion 731-2.
In utero cleft lip repairs were done by full-thickness approximation of the cleft edges by 11-0 sutures in the A/J mouse on day 17 of gestation after Dilantin was administered on day 10. On day 20, a cesarean section was performed, the repair inspected, and the lip sectioned for histologic study. One-thousand one-hundred and thirty-nine mice were bred, and surgery was performed on 48. At least one fetus was present that had a cleft lip in 21 mice, and repairs were done on 16 fetuses. Ultimately, 9 viable fetuses were studied. In all cases, lip continuity was present where the suture had coapted the edges. There was little or no evidence of the lip repair despite no incision being performed. Histologic examination revealed epithelial and mesenchymal continuity with an occasional notch noted in the epithelium and soft-tissue asymmetry in complete clefts. The implications of these findings are discussed.
在妊娠第10天给予苯妥英钠后，于妊娠第17天在A/J小鼠中通过用11-0缝线全层拉拢腭裂边缘进行宫内唇裂修复。在第20天，进行剖宫产，检查修复情况，并将唇部切片进行组织学研究。共繁殖了1139只小鼠，对48只进行了手术。21只小鼠中至少有1只胎儿患有唇裂，对16只胎儿进行了修复。最终，对9只存活胎儿进行了研究。在所有病例中，缝线拉拢边缘处唇连续性存在。尽管未进行切口，但几乎没有唇修复的证据。组织学检查显示上皮和间充质连续性，上皮偶尔有切迹，完全性腭裂存在软组织不对称。讨论了这些发现的意义。
