Stern M, Schmidt B, Dodson T B, Stern R, Kaban L B
Department of Oral and Maxillofacial Surgery, University of California, San Francisco 94143-0440.
J Oral Maxillofac Surg. 1992 Mar;50(3):263-8; discussion 269. doi: 10.1016/0278-2391(92)90323-r.
This study examines the histologic and biochemical features of wound healing in a cleft lip model in the mid-third-trimester fetal rabbit. At days 1, 2, and 4 after the procedure, control, unrepaired, and repaired fetal heads were obtained, sectioned, and stained for histologic examination. The localization of hyaluronic acid in the wound was documented using a cartilage-derived hyaluronic acid-binding protein. In both repaired and unrepaired wounds, the fetal cleft healed without inflammatory cell infiltration or scar formation. Six months after birth, the repaired cleft showed complete regeneration of muscle across the wound and the collagen fibers were of normal density and orientation. Decreased hyaluronic acid deposition was observed in unrepaired clefts as compared with adjacent tissue; no such difference was detected in repaired clefts. Our findings support the hypothesis that a cleft lip repaired in utero heals without the scarring that accompanies postnatal repair. This may explain the lack of maxillary growth restriction after in utero cleft lip repair.
本研究检测妊娠中期胎儿兔唇裂模型中伤口愈合的组织学和生化特征。在手术操作后第1、2和4天，获取对照、未修复和已修复的胎儿头部，进行切片并染色以进行组织学检查。使用软骨来源的透明质酸结合蛋白记录伤口中透明质酸的定位。在已修复和未修复的伤口中，胎儿唇裂均在无炎症细胞浸润或瘢痕形成的情况下愈合。出生6个月后，已修复的唇裂显示伤口处肌肉完全再生，胶原纤维密度和方向正常。与相邻组织相比，未修复的唇裂中透明质酸沉积减少；在已修复的唇裂中未检测到这种差异。我们的研究结果支持以下假设：子宫内修复的唇裂愈合时不会出现出生后修复所伴随的瘢痕形成。这可能解释了子宫内唇裂修复后上颌生长受限缺乏的原因。
