Mutlu Oguz, Ulak Guner, Kokturk Sibel, Komsuoglu Celikyurt Ipek, Tanyeri Pelin, Akar Furuzan, Erden Faruk
Department of Pharmacology, Medical Faculty, Kocaeli University, 41380, Kocaeli, Turkey.
Department of Histology and Embryology, Medical Faculty, Ordu University, 52000, Ordu, Turkey.
Homeopathy. 2016 Feb;105(1):96-101. doi: 10.1016/j.homp.2015.07.004. Epub 2015 Aug 24.
Homeopathy is a form of alternative medicine in which uses highly diluted preparations that are believed to cause healthy people to exhibit symptoms similar to those exhibited by patients. The aim of this study was to investigate the effects of dragonfly (Anax imperator, Anax i.) on learning and memory in naive mice using the Morris water maze (MWM) test; moreover, the effects of dragonfly on MK-801-induced cognitive dysfunction were evaluated.
Male balb-c mice were treated with dragonfly (30C and 200C) or MK-801 (0.2 mg/kg) alone or concurrently (n = 10). Dragonfly (D) and MK-801 were administered subchronically for 6 days intraperitoneally 60 min and 30 min, respectively, before the daily performance of the MWM test.
This study revealed that in the familiarization session and first session of the MWM test, Anax i. D30 significantly decreased escape latency compared to the control group, although MK-801, D30 and D200 significantly increased escape latency at the end of five acquisition sessions. Anax i. combined with dizocilpine maleate (MK-801) also significantly decreased escape latency in the familiarization session and first session of the MWM test, although this combination increased escape latency compared to the MK-801 alone group at the end of the test. Time spent in escape platform's quadrant in the probe trial significantly decreased while mean distance to platform significantly increased in MK-801, D30 and D200 groups. In the MWM test, Anax i. combined with MK-801 significantly decreased speed of the animals compared to the MK-801 alone group. General cell morphology was disturbed in the MK-801 group while D30 and D200 seemed to improve cell damage in the MK-801 group.
These results suggest that the homeopathic Anax i. can impair learning acquisition and reference memory, and it has beneficial effects on disturbed cell morphology.
顺势疗法是一种替代医学形式，它使用高度稀释的制剂，据信这些制剂会使健康人出现与患者相似的症状。本研究的目的是使用莫里斯水迷宫（MWM）试验研究蜻蜓（大斑巨脉蜓，大斑巨脉蜓）对未接触过该物质的小鼠学习和记忆的影响；此外，还评估了蜻蜓对MK-801诱导的认知功能障碍的影响。
雄性balb-c小鼠单独或同时接受蜻蜓（30C和200C）或MK-801（0.2mg/kg）处理（n = 10）。在每天进行MWM试验前60分钟和30分钟，分别腹腔内给予蜻蜓（D）和MK-801，连续6天。
本研究表明，在MWM试验的熟悉阶段和第一阶段，与对照组相比，大斑巨脉蜓D30显著降低了逃避潜伏期，尽管在五个训练阶段结束时，MK-801、D30和D200显著增加了逃避潜伏期。大斑巨脉蜓与马来酸氯氮平（MK-801）联合使用在MWM试验的熟悉阶段和第一阶段也显著降低了逃避潜伏期，尽管在试验结束时，与单独使用MK-801组相比，这种联合使用增加了逃避潜伏期。在探索试验中，MK-801、D30和D200组在逃避平台象限花费的时间显著减少，而到平台的平均距离显著增加。在MWM试验中，与单独使用MK-801组相比，大斑巨脉蜓与MK-801联合使用显著降低了动物的速度。MK-801组的一般细胞形态受到干扰，而D30和D200似乎改善了MK-801组的细胞损伤。
这些结果表明，顺势疗法的大斑巨脉蜓会损害学习获得和参考记忆，并且对受干扰的细胞形态有有益影响。
