Kettle Andrew J, Carere Jason, Batley Jacqueline, Manners John M, Kazan Kemal, Gardiner Donald M
Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia; University of Queensland (UQ), School of Agriculture and Food Sciences, St. Lucia, Queensland 4067, Australia.
Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture, Queensland Bioscience Precinct, Brisbane, Queensland 4067, Australia.
Fungal Genet Biol. 2016 Mar;88:44-53. doi: 10.1016/j.fgb.2016.01.015. Epub 2016 Jan 29.
A number of cereals produce the benzoxazolinone class of phytoalexins. Fusarium species pathogenic towards these hosts can typically degrade these compounds via an aminophenol intermediate, and the ability to do so is encoded by a group of genes found in the Fusarium Detoxification of Benzoxazolinone (FDB) cluster. A zinc finger transcription factor encoded by one of the FDB cluster genes (FDB3) has been proposed to regulate the expression of other genes in the cluster and hence is potentially involved in benzoxazolinone degradation. Herein we show that Fdb3 is essential for the ability of Fusarium pseudograminearum to efficiently detoxify the predominant wheat benzoxazolinone, 6-methoxy-benzoxazolin-2-one (MBOA), but not benzoxazoline-2-one (BOA). Furthermore, additional genes thought to be part of the FDB gene cluster, based upon transcriptional response to benzoxazolinones, are regulated by Fdb3. However, deletion mutants for these latter genes remain capable of benzoxazolinone degradation, suggesting that they are not essential for this process.
许多谷物会产生苯并恶唑啉酮类植保素。对这些宿主致病的镰刀菌通常可通过氨基酚中间体降解这些化合物，而这样做的能力由镰刀菌苯并恶唑啉酮解毒（FDB）簇中的一组基因编码。FDB簇基因之一（FDB3）编码的一种锌指转录因子被认为可调节簇中其他基因的表达，因此可能参与苯并恶唑啉酮的降解。在此我们表明，Fdb3对于假禾谷镰刀菌有效解毒主要的小麦苯并恶唑啉酮6-甲氧基-苯并恶唑啉-2-酮（MBOA）的能力至关重要，但对苯并恶唑啉-2-酮（BOA）则不然。此外，基于对苯并恶唑啉酮的转录反应而被认为是FDB基因簇一部分的其他基因受Fdb3调控。然而，这些后一类基因的缺失突变体仍能够降解苯并恶唑啉酮，这表明它们对于此过程并非必不可少。
