Chen Jieping, Zhu Xiashi
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
Food Chem. 2016 Jun 1;200:10-5. doi: 10.1016/j.foodchem.2016.01.002. Epub 2016 Jan 2.
Three hydrophobic ionic liquids (ILs) (1-butyl-3-methylimidazole hexafluorophosphate ([BMIM]PF6), 1-hexyl-3-methyl-imidazole hexafluoro-phosphate ([HMIM]PF6), and 1-octyl-3-methylimidazole hexafluorophosphate ([OMIM]PF6)) were used to coat Fe3O4@SiO2 nanoparticles (NPs) with core-shell structures to prepare magnetic solid phase extraction (MSPE) agents (Fe3O4@SiO2@IL). A novel method of MSPE coupled with high-performance liquid chromatography for the separation/analysis of Rhodamine B was then established. The results showed that Rhodamine B was adsorbed rapidly on Fe3O4@SiO2@[OMIM]PF6 and was released using ethanol. Under optimal conditions, the pre-concentration factor for the proposed method was 25. The linear range, limit of detection (LOD), correlation coefficient (R), and relative standard deviation (RSD) were found to be 0.50-150.00 μgL(-1), 0.08 μgL(-1), 0.9999, and 0.51% (n=3, c=10.00 μgL(-1)), respectively. The Fe3O4@SiO2 NPs could be re-used up to 10 times. The method was successfully applied to the determination of Rhodamine B in food samples.
使用三种疏水性离子液体（ILs）（1-丁基-3-甲基咪唑六氟磷酸盐（[BMIM]PF6）、1-己基-3-甲基咪唑六氟磷酸盐（[HMIM]PF6）和1-辛基-3-甲基咪唑六氟磷酸盐（[OMIM]PF6））包覆具有核壳结构的Fe3O4@SiO2纳米颗粒（NPs），以制备磁性固相萃取（MSPE）剂（Fe3O4@SiO2@IL）。然后建立了一种将MSPE与高效液相色谱联用的新方法，用于罗丹明B的分离/分析。结果表明，罗丹明B能迅速吸附在Fe3O4@SiO2@[OMIM]PF6上，并能用乙醇洗脱。在最佳条件下，该方法的预富集因子为25。线性范围、检测限（LOD）、相关系数（R）和相对标准偏差（RSD）分别为0.50 - 150.00 μg L(-1)、0.08 μg L(-1)、0.9999和0.51%（n = 3，c = 10.00 μg L(-1)）。Fe3O4@SiO2 NPs可重复使用多达10次。该方法成功应用于食品样品中罗丹明B的测定。
