Badragheh Sahar, Zeeb Mohsen, Talei Bavil Olyai Mohamad Reza
Department of Chemistry, Karaj Branch, Islamic Azad University Karaj Iran.
Department of Applied Chemistry, Faculty of Science, Islamic Azad University, South Tehran Branch Tehran Iran
RSC Adv. 2018 Aug 29;8(53):30550-30561. doi: 10.1039/c8ra02109k. eCollection 2018 Aug 24.
Herein, silica-coated iron oxide nanoparticles modified with imidazolium-based polymeric ionic liquid (FeO@SiO@PIL) were fabricated as a sustainable sorbent for magnetic solid-phase extraction (MSPE) and simultaneous determination of trace antidiabetic drugs in human plasma by high-performance liquid chromatography-ultraviolet detection (HPLC-UV). The FeO core was functionalized by silica (SiO) and vinyl layers where the ionic liquid 1-vinyl-3-octylimidazolium bromide (VOIM-Br) was attached through a free radical copolymerization process. In order to achieve hydrophobic magnetic nanoparticles and increase the merits of the sorbent, Br anions were synthetically replaced with PF . The properties and morphology of the sorbent were characterized by various techniques and all the results illustrated the prosperous synthesis of FeO@SiO@PIL. A comprehensive study was carried out to investigate and optimize various parameters affecting the extraction efficiency. The limit of detection (LOD, S/N = 3) for empagliflozin, metformin and canagliflozin was 1.3, 6.0 and 0.8 ng mL, respectively. Linearity (0.997 ≥ ≥ 0.993) and linear concentration ranges of 5.0-1200.0, 20.0-1800.0 and 5.0-1000.0 ng mL were obtained for empagliflozin, metformin and canagliflozin, respectively. Intra-assay (3.8-7.5%, = 9) and inter-assay (3.2-8.5%, = 12) precisions as well as accuracies (≤9.1%) displayed good efficiency of the method. Finally, the method was applied for the quantitation of antidiabetic drugs in human plasma after oral administration and main pharmacokinetic data including (h), (ng mL), AUC (ng h mL), AUC (ng h mL), and (h) were evaluated.
在此，制备了用咪唑基聚合离子液体修饰的二氧化硅包覆氧化铁纳米颗粒（FeO@SiO@PIL），作为一种可持续的吸附剂用于磁性固相萃取（MSPE），并通过高效液相色谱 - 紫外检测（HPLC - UV）同时测定人血浆中的痕量抗糖尿病药物。FeO核通过二氧化硅（SiO）和乙烯基层进行功能化，其中离子液体1 - 乙烯基 - 3 - 辛基咪唑溴化物（VOIM - Br）通过自由基共聚过程连接。为了获得疏水性磁性纳米颗粒并提高吸附剂的性能，用PF合成取代Br阴离子。通过各种技术对吸附剂的性质和形态进行了表征，所有结果表明成功合成了FeO@SiO@PIL。进行了全面研究以考察和优化影响萃取效率的各种参数。恩格列净、二甲双胍和卡格列净的检测限（LOD，S/N = 3）分别为1.3、6.0和0.8 ng/mL。恩格列净、二甲双胍和卡格列净的线性度（0.997≥r≥0.993）和线性浓度范围分别为5.0 - 1200.0、20.0 - 1800.0和5.0 - 1000.0 ng/mL。批内精密度（3.8 - 7.5%，n = 9）和批间精密度（3.2 - 8.5%，n = 12）以及准确度（≤9.1%）表明该方法具有良好的效率。最后，该方法用于口服给药后人血浆中抗糖尿病药物的定量分析，并评估了主要药代动力学数据，包括tmax（h）、Cmax（ng/mL）、AUC0 - t（ng·h/mL）、AUC0 - ∞（ng·h/mL）和t1/2（h）。
