Shi Jing-Hua, Jin Li, Leng Jin-Hua, Lang Jing-He
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Chin Med J (Engl). 2016 Jan 20;129(2):200-5. doi: 10.4103/0366-6999.173491.
Adenomyosis (AM) has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells (MSMCs) of AM.
Uterine tissue samples from 22 patients (cases) with histologically confirmed AM and 12 (controls) with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium- and voltage-sensitive K + channel (BKCa)-α/β subunits, voltage-gated potassium channel (Kv) 4.2, and Kv4.3. Student's t-test was used to compare the expression.
The BKCa-α/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-α messenger RNA (mRNA) (0.62 ± 0.19-fold decrease, P < 0.05) and Kv4.3 mRNA (0.67 ± 0.20-fold decrease, P < 0.05) decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA.
The BKCa-α mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.
子宫腺肌病（AM）存在收缩功能受损的情况。本研究旨在探讨AM患者子宫肌层平滑肌细胞（MSMCs）中与收缩相关的钾通道表达。
收集22例经组织学确诊为AM的患者（病例组）和12例宫颈上皮内瘤变患者（对照组）的子宫组织样本，进行免疫组织化学和实时聚合酶链反应，以检测大电导钙和电压敏感性钾通道（BKCa）-α/β亚基、电压门控钾通道（Kv）4.2和Kv4.3的表达。采用学生t检验比较表达情况。
BKCa-α/β亚基、Kv4.2和Kv4.3定位于平滑肌细胞、腺上皮和基质细胞。然而，对照组子宫内膜腺体中BKCa-β亚基表达较弱，对照组中Kv4.3几乎检测不到。与AM组相比，对照组MSMCs中BKCa-α信使核糖核酸（mRNA）表达显著降低（降低0.62±0.19倍，P<0.05），Kv4.3 mRNA表达显著降低（降低0.67±0.20倍，P<0.05）。然而，BKCa-β亚基mRNA或Kv4.2 mRNA无显著差异。
AM中BKCa-α mRNA和Kv4.3 mRNA的表达显著高于对照组，这可能导致子宫平滑肌收缩异常，改变子宫微循环以积聚炎症因子，进一步损害子宫内膜，并加重疼痛。
