Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Fudan University, Shanghai 200011, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5370-5376. doi: 10.3892/mmr.2017.7238. Epub 2017 Aug 14.
Adenomyosis is a common gynecologic disorder characterized by the presence of endometrial glands and stroma within the myometrium. The present study investigated the effect of quercetin in neonatal Imprinting Control Region mice with tamoxifen‑induced adenomyosis. The body weight and hotplate response latency of all mice was examined at 4, 8, 12 and 16 weeks after birth. The mice dosed with tamoxifen were divided into four groups: high‑ or low‑quercetin group, valproic acid (VPA) group and untreated group. The group of mice that were neonatally administrated with the solvent only (no tamoxifen), received no treatment and served as a blank control group. After 3 weeks of drug treatment, the potential ability of quercetin to improve the generalized hyperalgesia in mice with induced adenomyosis was evaluated by determining the body weight, pain modulation, examining the myometrial infiltration by histology examination of the uterus and detecting the expression of transient receptor potential cation channel subfamily V member 1 (Trpv‑1), phospho (p)‑p38 mitogen activated protein kinase p‑extracellular signal‑regulated kinase (p‑ERK) in DRG neurons via immunohistochemistry. The results demonstrated that treatment with quercetin improved the generalized hyperalgesia by extending the hotplate response latency, reduced myometrial infiltration and decreased the expression levels Trpv‑1, p‑p38 and p‑ERK in dorsal root ganglion neurons. The results indicated that quercetin decreases the incidence of hyperalgesia in mice with tamoxifen‑induced adenomyosis, and the potential mechanism is through reduced central sensitization, which may be a promising treatment for adenomyosis.
子宫腺肌病是一种常见的妇科疾病，其特征是子宫内膜腺体和基质存在于子宫肌层中。本研究探讨了槲皮素在他莫昔芬诱导的腺肌病新生印迹控制区小鼠中的作用。在出生后 4、8、12 和 16 周时，检查所有小鼠的体重和热板反应潜伏期。用他莫昔芬给药的小鼠分为四组：高或低槲皮素组、丙戊酸（VPA）组和未处理组。仅在新生儿期给予溶剂（无他莫昔芬）的小鼠组未接受任何治疗，作为空白对照组。药物治疗 3 周后，通过测定体重、疼痛调节、组织学检查子宫肌层浸润以及检测瞬时受体电位阳离子通道亚家族 V 成员 1（Trpv-1）、磷酸化（p）-p38 丝裂原激活蛋白激酶 p-细胞外信号调节激酶（p-ERK）在背根神经节神经元中的表达，评估槲皮素改善诱导的腺肌病小鼠全身痛觉过敏的潜在能力。结果表明，槲皮素通过延长热板反应潜伏期改善了全身痛觉过敏，减少了子宫肌层浸润，并降低了背根神经节神经元中 Trpv-1、p-p38 和 p-ERK 的表达水平。结果表明，槲皮素可降低他莫昔芬诱导的腺肌病小鼠的痛觉过敏发生率，其潜在机制可能是通过减少中枢敏化，这可能是治疗腺肌病的一种有前途的方法。
