Wang Caixia, Wang Yibo, Paterson James S, Mitchell James G, Hu Xiaoke, Zhang Hua, Sheng Yanqing
Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China University of Chinese Academy of Sciences, Beijing 100049, China.
School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
FEMS Microbiol Ecol. 2016 Mar;92(3). doi: 10.1093/femsec/fiw017. Epub 2016 Jan 31.
In light of limited research into the relationship between the macroscale distribution and dynamic changes of microplankton in the shallow Bohai Sea, here we used flow cytometry to analyse samples collected from the Bohai Sea channel in winter and summer. Results showed that the average of both viral abundance (VA) and bacterial abundance (BA) were lower in winter (3.61 × 10(7) and 1.84 × 10(6) cells/mL, respectively) than in summer (7.47 × 10(7) and 5.05 × 10(6) cells/mL, respectively). At all 16 stations, VA was one order of magnitude greater than BA, with a positive relationship between one another. In the horizontal distribution, variations in VA and BA followed a similar trend, and both were obviously higher near-shore than offshore. In the vertical distribution, variations in both VA and BA did not show a clear relationship with water depth. VA and BA in summer were 2.1 and 2.7 times those in winter, respectively. Spearman correlation analysis showed that both VA and BA were correlated with the concentration of PO4-P in winter (positive) and NO3-N in summer (negative). Additionally, BA showed a negative correlation with salinity. It is clear that the macroscale distribution of these two kinds of microbes in the Bohai Sea is related to seasonal variation and nutrient availability.
鉴于对渤海浅水区微型浮游生物的宏观分布与动态变化之间关系的研究有限，我们在此使用流式细胞仪分析了冬季和夏季从渤海海峡采集的样本。结果表明，冬季的病毒丰度（VA）和细菌丰度（BA）平均值（分别为3.61×10⁷和1.84×10⁶个细胞/毫升）均低于夏季（分别为7.47×10⁷和5.05×10⁶个细胞/毫升）。在所有16个站点，VA比BA高一个数量级，且二者呈正相关。在水平分布上，VA和BA的变化趋势相似，且近岸处均明显高于离岸处。在垂直分布上，VA和BA的变化与水深均无明显关系。夏季的VA和BA分别是冬季的2.1倍和2.7倍。Spearman相关性分析表明，VA和BA在冬季均与PO4-P浓度呈正相关，在夏季均与NO3-N浓度呈负相关。此外，BA与盐度呈负相关。显然，渤海这两种微生物的宏观分布与季节变化和养分有效性有关。
