Center for Medical Mycology, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, OH, USA.
Galderma R & D SNC, Sophia Antipolis, France.
Dermatol Ther (Heidelb). 2016 Mar;6(1):69-75. doi: 10.1007/s13555-016-0093-x. Epub 2016 Feb 1.
Onychomycosis is the most common infectious disease involving nails. The aim of this study was to evaluate the antifungal activity of amorolfine 5% nail lacquer and three different acid-based medical devices indicated in the treatment of onychomycosis using an in vitro nail penetration assay.
Four products were tested in vitro: (a) amorolfine 5% nail lacquer; (b) ethyl lactate and acetic acid; (c) citric acid and urea; (d) ethyl lactate, glycerin, lactic acid, and citric acid. Test products were applied to healthy human cadaver nails and allowed to dry. Disks were cut from each piece of nail and placed on seeded agar plates of Trichophyton rubrum. Following incubation at 30 °C, zones of inhibition were measured.
Amorolfine-treated nails exhibited inhibitory activity against T. rubrum with a mean zone of inhibition of 59.2 mm in diameter. In contrast, all three acid-based medical devices and the untreated controls showed no zones of inhibition (mean effective zones of 0 mm).
In this in vitro nail penetration model, head-on, comparative study, we showed that amorolfine 5% nail lacquer possesses potent antifungal activity, whereas no antifungal activity was detected for three commercially available acid-based medical devices under identical assay conditions.
Galderma.
甲真菌病是最常见的指甲感染性疾病。本研究旨在使用体外指甲渗透测定法评估阿莫罗芬 5%指甲油和三种不同的基于酸的医疗设备在治疗甲真菌病方面的抗真菌活性。
在体外测试了四种产品：（a）阿莫罗芬 5%指甲油；（b）乳酸乙酯和乙酸；（c）柠檬酸和尿素；（d）乳酸乙酯、甘油、乳酸和柠檬酸。将测试产品应用于健康的人体尸体指甲上并使其干燥。从每片指甲上切下圆盘，并将其放在接种有红色毛癣菌的琼脂平板上。在 30°C 孵育后，测量抑菌环的大小。
阿莫罗芬处理的指甲对 T. rubrum 表现出抑制活性，平均抑菌环直径为 59.2 毫米。相比之下，所有三种基于酸的医疗设备和未处理的对照均未显示抑菌环（平均有效抑菌环为 0 毫米）。
在这项体外指甲渗透模型的直接比较研究中，我们表明阿莫罗芬 5%指甲油具有强大的抗真菌活性，而在相同的检测条件下，三种市售的基于酸的医疗设备均未检测到抗真菌活性。
高德美。
