Hultenby Kjell, Chryssanthou Erja, Klingspor Lena, Rensfeldt Kjell, Strömbeck Louise, Faergemann Jan
Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
Mycoses. 2014 Oct;57(10):630-8. doi: 10.1111/myc.12211. Epub 2014 Jun 17.
K101 Nail Solution (trademarks Emtrix(®), Nalox(™), Naloc(™)) is a combination of propylene glycol, urea and lactic acid in a topical formulation for the treatment of nails affected by onychomycosis. The aim of this study was to investigate the Minimal Cidal Concentration (MCC) of K101 Nail Solution against Trichophyton rubrum and Candida albicans as well as the effect of K101 Nail Solution on the micromorphology of these fungi. The MCC of K101 Nail Solution against T. rubrum and C. albicans was 50% after 60-min exposure time. A MCC of 50% for K101 Nail Solution means that K101 Nail Solution diluted with e.g. water to 50% will totally kill the fungi tested. In the scanning electron microscope C. albicans cells, treated with 50% K101 Nail Solution, showed a shrunken surface. T. rubrum cells were severely damaged shown as collapse and degradation of the cells. In the transmission electron microscope most C. albicans cells, treated with 50% K101 Nail Solution exhibited destroyed organelles and many necrotic cells were found. The cell wall was clearly degraded and the contact between the cell wall and the inner membrane was punctured. In T. rubrum most cells were necrotic. Some cells were clearly collapsed and the content in the cytoplasm was degraded shown as small membrane vesicles and many big vacuoles. The cell wall was clearly degraded and the membrane was punctured. In conclusion, this in vitro study documents the efficacy of K101 Nail Solution against T. rubrum and C. albicans.
K101指甲溶液（商标为Emtrix(®)、Nalox(™)、Naloc(™)）是一种局部制剂，由丙二醇、尿素和乳酸组成，用于治疗受甲癣影响的指甲。本研究的目的是调查K101指甲溶液对红色毛癣菌和白色念珠菌的最低杀菌浓度（MCC），以及K101指甲溶液对这些真菌微观形态的影响。K101指甲溶液在暴露60分钟后对红色毛癣菌和白色念珠菌的MCC为50%。K101指甲溶液的MCC为50%意味着用例如水稀释至50%的K101指甲溶液将完全杀死所测试的真菌。在扫描电子显微镜下，用50%K101指甲溶液处理的白色念珠菌细胞表面收缩。红色毛癣菌细胞严重受损，表现为细胞塌陷和降解。在透射电子显微镜下，大多数用50%K101指甲溶液处理的白色念珠菌细胞细胞器被破坏，发现许多坏死细胞。细胞壁明显降解，细胞壁与内膜之间的接触被刺破。在红色毛癣菌中，大多数细胞坏死。一些细胞明显塌陷，细胞质中的内容物降解，表现为小膜泡和许多大液泡。细胞壁明显降解，细胞膜被刺破。总之，这项体外研究证明了K101指甲溶液对红色毛癣菌和白色念珠菌的有效性。
