Rocha R M P, Lima L F, Carvalho A A, Chaves R N, Bernuci M P, Rosa-e-Silva A C J S, Rodrigues A P R, Campello C C, Figueiredo J R
Laboratory of Manipulation of Oocytes and Preantral Follicles- LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza-CE, Brazil.
Laboratory Morphofunctional, Health Sciences Center, University of Fortaleza, Fortaleza-CE, Brazil.
Reprod Domest Anim. 2016 Apr;51(2):212-9. doi: 10.1111/rda.12668. Epub 2016 Feb 2.
The aims of this study were to evaluate the localization, by immunohistochemistry, of the anti-Müllerian hormone (AMH) in goat ovaries and to investigate its effects on the in vitro survival and development of caprine pre-antral follicles enclosed in fragments of ovarian tissue. Pre-antral follicles were cultured in vitro for 1 or 7 days in α-MEM(+) in the absence or presence of kit ligand (KL; 50 ng/ml, positive control) or AMH (50 or 150 ng/ml). The results showed that AMH was localized in oocytes and granulosa cells from the primordial follicle to antral follicle stages. Addition of AMH maintained the percentage of developing follicles, similar to that in the uncultured control; however, the percentage of developing follicles was significantly lower than that in the cultured control and KL. Nonetheless, addition of AMH to the culture medium did not affect survival rates and follicular growth. In conclusion, this study demonstrated that the expression of AMH varies according to the compartment and stage of follicular development. Furthermore, AMH inhibits the activation of caprine primordial follicles.
本研究的目的是通过免疫组织化学评估抗苗勒管激素（AMH）在山羊卵巢中的定位，并研究其对包埋于卵巢组织片段中的山羊腔前卵泡体外存活和发育的影响。将腔前卵泡在不含或含有试剂盒配体（KL；50 ng/ml，阳性对照）或AMH（50或150 ng/ml）的α-MEM(+)中体外培养1天或7天。结果显示，从原始卵泡到窦卵泡阶段，AMH定位于卵母细胞和颗粒细胞中。添加AMH可维持发育卵泡的百分比，与未培养的对照组相似；然而，发育卵泡的百分比显著低于培养对照组和KL组。尽管如此，向培养基中添加AMH并不影响存活率和卵泡生长。总之，本研究表明AMH的表达随卵泡发育的区域和阶段而变化。此外，AMH抑制山羊原始卵泡的激活。
