Gautam Devika, Vats Ashutosh, Pal Prasanna, Haldar Avijit, De Sachinandan
Animal Genomics Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (NDRI), Karnal, India.
Animal Physiology Division, ICAR-National Dairy Research Institute (NDRI), Karnal, India.
Front Vet Sci. 2021 Mar 8;8:627094. doi: 10.3389/fvets.2021.627094. eCollection 2021.
The Anti-Müllerian Hormone (AMH) is a member of the transforming growth factor beta (TGF-β) superfamily, playing a significant role in cell proliferation, differentiation and apoptosis. In females, AMH is secreted throughout their reproductive life span from ovaries, whereas in males it is secreted by gonadal cells at a very early stage of testicular development. AMH is a promising marker of ovarian reserve in women and can be used to measure the female reproductive lifespan. In the present study, we cloned and sequenced the GC rich gene from Indian riverine buffalo ( and goat (). Obtained sequences were compared to the AMH sequences of other mammals, and corresponding amino acid sequences revealed that the caprine and bovine AMH sequences are more closely related to each other than to those of other mammals. Furthermore, we analyzed the chromosomal localization of genes in mammalian species to understand potential syntenic relationship. The gene is localized between the sequences for the and genes and maintains this precise location in relation to other nearby genes. The dN/dS ratio of gene did not indicate any pressure for either positive or negative selection; thus, the physiological function of the gene in the reproduction of these two ruminant species remains very vital. Similar to other mammals, the gene may be an important indicator for regulating female reproductive biology function in bovine, cetacean, caprine, and camelidae.
抗苗勒管激素（AMH）是转化生长因子β（TGF-β）超家族的成员，在细胞增殖、分化和凋亡中发挥重要作用。在雌性动物中，AMH在其整个生殖寿命期间由卵巢分泌，而在雄性动物中，它在睾丸发育的非常早期阶段由性腺细胞分泌。AMH是女性卵巢储备的一个有前景的标志物，可用于衡量女性的生殖寿命。在本研究中，我们从印度河流水牛（和山羊（）中克隆并测序了富含GC的基因。将获得的序列与其他哺乳动物的AMH序列进行比较，相应的氨基酸序列显示，山羊和牛的AMH序列彼此之间的关系比与其他哺乳动物的关系更密切。此外，我们分析了哺乳动物物种中基因的染色体定位，以了解潜在的同线性关系。该基因位于和基因的序列之间，并相对于其他附近基因保持这一精确位置。基因的dN/dS比值未显示出任何正选择或负选择的压力；因此，该基因在这两种反刍动物繁殖中的生理功能仍然非常重要。与其他哺乳动物类似，该基因可能是调节牛、鲸类、山羊和骆驼科动物雌性生殖生物学功能的重要指标。
