Spendier Kathrin
BioFrontiers Center and Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.
Results Immunol. 2015 Dec 3;6:1-4. doi: 10.1016/j.rinim.2015.11.001. eCollection 2016.
Biomembranes undergo extensive shape changes as they perform vital cellular functions or become diseased. To understand the mechanisms by which lipids and proteins control membrane curvature during various processes, researchers have identified and engineered many curvature-inducing and curvature-sensing proteins and peptides. In this paper, a simple experiment was performed to show qualitatively how membrane remodeling by N-terminal amphipathic helix of Amphiphysin affects the spatial distribution of the transmembrane Fc receptor protein (FcεRI) in mast cells. Results indicate that an elevated concentration of amphipathic helices can interfere with the formation of a typical mast cell synapse.
生物膜在执行重要细胞功能或发生病变时会经历广泛的形状变化。为了解脂质和蛋白质在各种过程中控制膜曲率的机制，研究人员已经鉴定并设计了许多诱导曲率和感知曲率的蛋白质及肽段。在本文中，我们进行了一个简单的实验，以定性地展示发动蛋白N端两亲性螺旋对膜的重塑如何影响肥大细胞中跨膜Fc受体蛋白（FcεRI）的空间分布。结果表明，两亲性螺旋浓度升高会干扰典型肥大细胞突触的形成。
