Tsai Ming Chih, Spendier Kathrin
Department of Physics and Energy Science, University of Colorado Colorado Springs, Colorado Springs, CO 80918, USA.
BioFrontiers Center, University of Colorado Colorado Springs, Colorado Springs, CO 80918, USA.
Biophysica. 2022 Dec;2(4):428-439. doi: 10.3390/biophysica2040038. Epub 2022 Nov 7.
The RBL-2H3 mast cell immunological synapse dynamics is often simulated with reaction-diffusion and Fokker-Planck equations. The equations focus on how the cell synapse captures receptors following an immune response, where the receptor capture at the immunological site appears to be a delayed process. This article investigates the physical nature and mathematics behind such time-dependent delays. Using signal processing methods, convolution and cross-correlation-type delay capture simulations give a -squared range of 22 to 60, in good agreement with experimental results. The cell polarization event is offered as a possible explanation for these capture delays, where polarizing rates measure how fast the cell polarization event occurs. In the case of RBL-2H3 mast cells, polarization appears to be associated with cytoskeletal rearrangement; thus, both cytoskeletal and diffusional components are considered. From these simulations, a maximum polarizing rate ranging from 0.0057 s to 0.031 s is obtained. These results indicate that RBL-2H3 mast cells possess both temporal and spatial memory, and cell polarization is possibly linked to a Turing-type pattern formation.
RBL - 2H3肥大细胞免疫突触动力学通常用反应扩散方程和福克 - 普朗克方程来模拟。这些方程关注的是细胞突触在免疫反应后如何捕获受体，其中免疫部位的受体捕获似乎是一个延迟过程。本文研究了这种时间依赖性延迟背后的物理本质和数学原理。使用信号处理方法，卷积和互相关类型的延迟捕获模拟给出的(R^2)范围为22至60，与实验结果高度吻合。细胞极化事件被认为是这些捕获延迟的一种可能解释，其中极化速率衡量细胞极化事件发生的速度。对于RBL - 2H3肥大细胞，极化似乎与细胞骨架重排有关；因此，细胞骨架和扩散成分都被考虑在内。从这些模拟中，获得了范围从0.0057 s至0.031 s的最大极化速率。这些结果表明RBL - 2H3肥大细胞具有时间和空间记忆，并且细胞极化可能与图灵型模式形成有关。
