Department of Chemistry and Pharmacy, University of Erlangen-Nuremberg , Erlangen , Germany.
Institute of Biochemistry, University of Erlangen-Nuremberg , Erlangen , Germany.
Front Bioeng Biotechnol. 2016 Jan 19;3:211. doi: 10.3389/fbioe.2015.00211. eCollection 2015.
The design and generation of molecules capable of mimicking the binding and/or functional sites of proteins represents a promising strategy for the exploration and modulation of protein function through controlled interference with the underlying molecular interactions. Synthetic peptides have proven an excellent type of molecule for the mimicry of protein sites because such peptides can be generated as exact copies of protein fragments, as well as in diverse chemical modifications, which includes the incorporation of a large range of non-proteinogenic amino acids as well as the modification of the peptide backbone. Apart from extending the chemical and structural diversity presented by peptides, such modifications also increase the proteolytic stability of the molecules, enhancing their utility for biological applications. This article reviews recent advances by this and other laboratories in the use of synthetic protein mimics to modulate protein function, as well as to provide building blocks for synthetic biology.
设计和生成能够模拟蛋白质结合和/或功能位点的分子，是通过控制对基础分子相互作用的干扰来探索和调节蛋白质功能的一种很有前途的策略。合成肽已被证明是一种极好的模拟蛋白质位点的分子类型，因为此类肽可以作为蛋白质片段的精确复制品生成，还可以进行多种化学修饰，包括掺入大量非天然氨基酸以及对肽骨架进行修饰。除了增加肽所呈现的化学和结构多样性外，这些修饰还提高了分子的蛋白水解稳定性，增强了它们在生物应用中的实用性。本文综述了本实验室和其他实验室在利用合成蛋白模拟物来调节蛋白质功能以及为合成生物学提供构建模块方面的最新进展。
