University of California, Irvine, Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States; University of California, Irvine, Department of Biomedical Engineering, 3120 Natural Sciences II, Irvine, California 92697-2715, United States.
University of California, Irvine , Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92612, United States.
Neurophotonics. 2015 Oct;2(4):045001. doi: 10.1117/1.NPh.2.4.045001. Epub 2015 Oct 12.
Baseline optical properties are typically assumed in calculating the differential pathlength factor (DPF) of mouse brains, a value used in the modified Beer-Lambert law to characterize an evoked stimulus response. We used spatial frequency domain imaging to measure in vivo baseline optical properties in 20-month-old control ([Formula: see text]) and triple transgenic APP/PS1/tau (3xTg-AD) ([Formula: see text]) mouse brains. Average [Formula: see text] for control and 3xTg-AD mice was [Formula: see text] and [Formula: see text], respectively, at 460 nm; and [Formula: see text] and [Formula: see text], respectively, at 530 nm. Average [Formula: see text] for control and 3xTg-AD mice was [Formula: see text] and [Formula: see text], respectively, at 460 nm; and [Formula: see text] and [Formula: see text], respectively, at 530 nm. The calculated DPF for control and 3xTg-AD mice was [Formula: see text] and [Formula: see text] OD mm, respectively, at 460 nm; and [Formula: see text] and [Formula: see text] OD mm, respectively, at 530 nm. In hindpaw stimulation experiments, the hemodynamic increase in brain tissue concentration of oxyhemoglobin was threefold larger and two times longer in the control mice compared to 3xTg-AD mice. Furthermore, the washout of deoxyhemoglobin from increased brain perfusion was seven times larger in controls compared to 3xTg-AD mice ([Formula: see text]).
基线光学特性通常用于计算小鼠大脑的差分路径长度因子（DPF），该因子用于在改良 Beer-Lambert 定律中对诱发刺激反应进行特征描述。我们使用空间频率域成像技术在 20 个月大的对照组（[Formula: see text]）和三转基因 APP/PS1/τ（3xTg-AD）（[Formula: see text]）小鼠脑中测量了体内基线光学特性。对照组和 3xTg-AD 小鼠的平均[Formula: see text]在 460nm 处分别为[Formula: see text]和[Formula: see text]，在 530nm 处分别为[Formula: see text]和[Formula: see text]。对照组和 3xTg-AD 小鼠的平均[Formula: see text]在 460nm 处分别为[Formula: see text]和[Formula: see text]，在 530nm 处分别为[Formula: see text]和[Formula: see text]。对照组和 3xTg-AD 小鼠的计算 DPF 在 460nm 处分别为[Formula: see text]和[Formula: see text]ODmm，在 530nm 处分别为[Formula: see text]和[Formula: see text]ODmm。在后爪刺激实验中，与 3xTg-AD 小鼠相比，对照组小鼠脑组织中氧合血红蛋白浓度的血液动力学增加了三倍，持续时间延长了两倍。此外，与 3xTg-AD 小鼠相比，对照组中增加的脑灌注引起的脱氧血红蛋白的清除量增加了七倍[Formula: see text]。
