Polgár K, Adány R, Abel G, Kappelmayer J, Muszbek L, Papp Z
Department of Obstetrics and Gynecology, University Medical School of Debrecen, Hungary.
Am J Hum Genet. 1989 Nov;45(5):786-92.
Culture of human amniotic-fluid cells from cases of fetal neural tube defects produces a population of rapidly adhering cells that were initially thought to be macrophages and later interpreted to be of neural origin. In this study double and triple labeling systems for the simultaneous detection of glial and macrophage differentiation marker antigens have been used to demonstrate that rapidly adhering cells cannot be considered a homogeneous population but instead represent two distinct cell types. One of these cell populations is of glial origin and shows specific staining for glial fibrillary acidic protein, while the other population is monocyte-derived macrophages which express marker antigens recognized by Leu M3, KiM7, and Dako antimacrophage monoclonal antibodies.
对患有胎儿神经管缺陷病例的人羊水细胞进行培养，会产生一群快速黏附细胞，这些细胞最初被认为是巨噬细胞，后来被解释为神经来源。在本研究中，使用了用于同时检测神经胶质和巨噬细胞分化标志物抗原的双重和三重标记系统，以证明快速黏附细胞不能被视为同质群体，而是代表两种不同的细胞类型。其中一类细胞群体源自神经胶质，对神经胶质纤维酸性蛋白呈特异性染色，而另一类细胞群体是单核细胞衍生的巨噬细胞，表达由Leu M3、KiM7和达科抗巨噬细胞单克隆抗体识别的标志物抗原。
