Sarkar S, Chang H C, Porreco R P, Jones O W
Am J Obstet Gynecol. 1980 Jan 1;136(1):67-72. doi: 10.1016/0002-9378(80)90566-9.
Cells from the amniotic fluid of fetuses with open neural tube defects (NTDs) have several abnormal characteristics of potential diagnostic value. The cell number in a unit volume of fluid is 10 to 100 times the normal range of cell count. The majority of cells adhere to tissue culture dishes within 24 hours of inoculation, instead of the 4 or 5 days required by normal amniotic fluid cells. The rapidly adhering cells morphologically resemble glial cells. Their Coulter volume is about twice the average seen in normal amniotic fluid--derived cells. This larger number of oversized, rapidly adhering cells is likely due to the continuous accumulation of neural cells in the amniotic fluid. We have detected the presence of the glial protein S-100 and the absence of the neuronal protein 14-3-2 by using a specific immunofluorescence assay. We conclude that fetuses with NTDs shed large numbers of glialike cells into the amniotic fluid where their detection may have diagnostic importance.
患有开放性神经管缺陷（NTDs）的胎儿羊水中的细胞具有若干具有潜在诊断价值的异常特征。单位体积羊水中的细胞数量是正常细胞计数范围的10到100倍。大多数细胞在接种后24小时内就附着于组织培养皿，而正常羊水细胞则需要4或5天。快速附着的细胞在形态上类似于神经胶质细胞。它们的库尔特体积约为正常羊水来源细胞平均体积的两倍。这种大量超大、快速附着的细胞可能是由于神经细胞在羊水中持续积累所致。我们通过使用特异性免疫荧光测定法检测到了神经胶质蛋白S-100的存在以及神经元蛋白14-3-2的缺失。我们得出结论，患有NTDs的胎儿会向羊水中释放大量类神经胶质细胞，对其进行检测可能具有诊断意义。
