Cremer M, Schachner M, Cremer T, Schmidt W, Voigtländer T
Hum Genet. 1981;56(3):365-70. doi: 10.1007/BF00274694.
We have investigated the origin of rapidly adhering (RA) cells in three cases of neural tube defects (two anencephali, one encephalocele). We were able to demonstrate the presence of glial fibrillary acidic (GFA) protein in variable percentages (4--80%) of RA cells cultured for 4--6 days by use of indirect immunofluorescence with GFA antiserum. Cells cultured from amniotic fluids of normal pregnancies and fetal fibroblasts were completely GFA protein negative. GFA protein is well established as a highly specific marker for astrocytes. Demonstration of astrocytes may prove to be a criterion of high diagnostic value for neural tube defects. The percentage of astrocytes decreased with increasing culture time, while the percentage of fibronectin positive cells increased both in amniotic fluid cell cultures from neural tube defects and normal pregnancies.
我们研究了3例神经管缺陷（2例无脑儿、1例脑膨出）中快速黏附（RA）细胞的起源。通过使用胶质纤维酸性蛋白（GFA）抗血清进行间接免疫荧光法，我们能够证实在培养4 - 6天的RA细胞中，GFA蛋白的存在比例各不相同（4% - 80%）。从正常妊娠羊水和胎儿成纤维细胞培养的细胞完全呈GFA蛋白阴性。GFA蛋白是一种已被充分确立的星形胶质细胞高度特异性标志物。星形胶质细胞的证实可能成为神经管缺陷高诊断价值的一个标准。在神经管缺陷和正常妊娠的羊水细胞培养中，星形胶质细胞的比例随培养时间增加而降低，而纤连蛋白阳性细胞的比例则增加。
