Kano K, Fjelde A, Milgrom F
J Immunol. 1977 Sep;119(3):945-9.
Lymphoid cells obtained from spleens of patients with lymphomas or leukemias were studied for the presence of heterophile (Paul-Bunnell (P-B)) antigen. A mixed agglutination (MA) test was established utilizing monolayers of cells attached to poly-L-lysine-coated wells of plastic U plates. After incubation of the monolayers with infectious mononeucleosis (IM) sera, indicator cells, sheep, or trypsinized bovine erythrocytes were added. The results were assessed according to sedimentation patterns of the indicator cells on the monolayers. Positive MA reactions were shown to be due to specific binding of P-B antibodies to the corresponding antigens on the spleen cells. Positive results were obtained with 15 of 37 spleens from patients with Hodgkin's disease, 5 of 8 lymphoma spleens, 4 of 15 chronic myelocytic leukemia spleens and 2 of 4 chronic lymphocytic leukemia spleens. Only 2 of 25 spleens from patients with various other diseases and 1 of 26 apparently normal thymus specimens gave positive results. This study confirmed demonstration of P-B antigen in lymphoma and leukemia by means of absorption experiments, which was reported previously.
对从淋巴瘤或白血病患者脾脏中获取的淋巴细胞进行研究，以检测嗜异性（保罗 - 邦内尔（P - B））抗原的存在。利用附着在塑料U型板聚L - 赖氨酸包被孔中的单层细胞建立了混合凝集（MA）试验。在用传染性单核细胞增多症（IM）血清孵育单层细胞后，加入指示细胞、绵羊或胰蛋白酶处理的牛红细胞。根据指示细胞在单层上的沉降模式评估结果。阳性MA反应显示是由于P - B抗体与脾细胞上相应抗原的特异性结合。霍奇金病患者的37个脾脏中有15个、8个淋巴瘤脾脏中有5个、15个慢性粒细胞白血病脾脏中有4个以及4个慢性淋巴细胞白血病脾脏中有2个获得阳性结果。患有各种其他疾病的患者的25个脾脏中只有2个以及26个明显正常的胸腺标本中有1个呈阳性结果。本研究通过吸收实验证实了先前报道的淋巴瘤和白血病中P - B抗原的存在。
