Yoshimatsu Takeshi, D'Orazi Florence D, Gamlin Clare R, Suzuki Sachihiro C, Suli Arminda, Kimelman David, Raible David W, Wong Rachel O
Department of Biological Structure, University of Washington, 1959 NE Pacific Street, Box 357420, Seattle, Washington 98195, USA.
Department of Physiology and Developmental Biology, Brigham Young University, 3048 LSB, Provo, Utah 84602, USA.
Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590.
Whether neurons can restore their original connectivity patterns during circuit repair is unclear. Taking advantage of the regenerative capacity of zebrafish retina, we show here the remarkable specificity by which surviving neurons reassemble their connectivity upon regeneration of their major input. H3 horizontal cells (HCs) normally avoid red and green cones, and prefer ultraviolet over blue cones. Upon ablation of the major (ultraviolet) input, H3 HCs do not immediately increase connectivity with other cone types. Instead, H3 dendrites retract and re-extend to contact new ultraviolet cones. But, if regeneration is delayed or absent, blue-cone synaptogenesis increases and ectopic synapses are made with red and green cones. Thus, cues directing synapse specificity can be maintained following input loss, but only within a limited time period. Further, we postulate that signals from the major input that shape the H3 HC's wiring pattern during development persist to restrict miswiring after damage.
在神经回路修复过程中，神经元能否恢复其原始的连接模式尚不清楚。利用斑马鱼视网膜的再生能力，我们在此展示了存活神经元在其主要输入再生时重新组装连接的显著特异性。H3水平细胞（HCs）通常避开红色和绿色视锥细胞，并且比起蓝色视锥细胞更喜欢紫外线视锥细胞。在主要（紫外线）输入被切除后，H3 HCs不会立即增加与其他视锥细胞类型的连接。相反，H3树突会缩回并重新延伸以接触新的紫外线视锥细胞。但是，如果再生延迟或不存在，蓝色视锥细胞的突触形成会增加，并与红色和绿色视锥细胞形成异位突触。因此，引导突触特异性的线索在输入丧失后可以维持，但仅在有限的时间段内。此外，我们推测在发育过程中塑造H3 HC布线模式的来自主要输入的信号会持续存在，以限制损伤后的错误布线。
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