Lee Jane-Dar, Yang Wen-Kai, Lee Ming-Huei
Division of Urology, Department of Surgery, Taichung Armed Forces General Hospital, Taichung, Taiwan, Republic of China.; Central Taiwan University of Science and Technology, Taichung, Taiwan, Republic of China.; National Defense Medical Center, Taipei, Taiwan, Republic of China.
Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, Republic of China.
J Korean Med Sci. 2016 Feb;31(2):280-5. doi: 10.3346/jkms.2016.31.2.280. Epub 2016 Jan 19.
Na(+)/K(+)-ATPase (NKA) is abundantly expressed in the basolateral membrane of epithelial cells, which is necessary for tight junction formation. The tight junction is an urothelial barrier between urine and the underlying bladder. Impairment of tight junctions allows migration of urinary solutes in patients with interstitial cystitis/painful bladder syndrome (IC/PBS). We evaluated NKA expression and activity in bladder samples from patients with IC/PBS. The study group consisted of 85 patients with IC/PBS, and the control group consisted of 20 volunteers. Bladder biopsies were taken from both groups. We determined the expression and distribution of NKA using NKA activity assays, immunoblotting, immunohistochemical staining, and immunofluorescent staining. The protein levels and activity of NKA in the study group were significantly lower than the control group (1.08 ± 0.06 vs. 2.39 ± 0.29 and 0.60 ± 0.04 vs. 1.81 ± 0.18 µmol ADP/mg protein/hour, respectively; P < 0.05). Additionally, immunofluorescent staining for detection of CK7, a marker of the bladder urothelium, predominantly colocalized with NKA in patients in the study group. Our results demonstrated the expression and activity of NKA were decreased in bladder biopsies of patients with IC/PBS. These findings suggest that NKA function is impaired in the bladders from patients with IC/PBS.
钠钾ATP酶（NKA）在上皮细胞的基底外侧膜中大量表达，这对于紧密连接的形成是必需的。紧密连接是尿液与下方膀胱之间的尿路上皮屏障。紧密连接受损会导致间质性膀胱炎/膀胱疼痛综合征（IC/PBS）患者尿液溶质迁移。我们评估了IC/PBS患者膀胱样本中NKA的表达和活性。研究组由85例IC/PBS患者组成，对照组由20名志愿者组成。两组均进行了膀胱活检。我们使用NKA活性测定、免疫印迹、免疫组织化学染色和免疫荧光染色来确定NKA的表达和分布。研究组中NKA的蛋白水平和活性显著低于对照组（分别为1.08±0.06对2.39±0.29以及0.60±0.04对1.81±0.18微摩尔ADP/毫克蛋白/小时；P<0.05）。此外，在研究组患者中，用于检测膀胱尿路上皮标志物CK7的免疫荧光染色主要与NKA共定位。我们的结果表明，IC/PBS患者膀胱活检中NKA的表达和活性降低。这些发现提示，IC/PBS患者膀胱中NKA功能受损。
