Li Xiang, Bi Zhenghong, Di Rong, Liang Peng, He Qiguang, Liu Wenbo, Miao Weiguo, Zheng Fucong
College of Environment and Plant Protection, Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Hainan University, No. 58, Renmin Avenue, Haikou 570228, China.
College of Agriculture, Hainan University, Haikou 570228, China.
Int J Mol Sci. 2016 Jan 29;17(2):181. doi: 10.3390/ijms17020181.
Powdery mildew is an important disease of rubber trees caused by Oidium heveae B. A. Steinmann. As far as we know, none of the resistance genes related to powdery mildew have been isolated from the rubber tree. There is little information available at the molecular level regarding how a rubber tree develops defense mechanisms against this pathogen. We have studied rubber tree mRNA transcripts from the resistant RRIC52 cultivar by differential display analysis. Leaves inoculated with the spores of O. heveae were collected from 0 to 120 hpi in order to identify pathogen-regulated genes at different infection stages. We identified 78 rubber tree genes that were differentially expressed during the plant-pathogen interaction. BLAST analysis for these 78 ESTs classified them into seven functional groups: cell wall and membrane pathways, transcription factor and regulatory proteins, transporters, signal transduction, phytoalexin biosynthesis, other metabolism functions, and unknown functions. The gene expression for eight of these genes was validated by qRT-PCR in both RRIC52 and the partially susceptible Reyan 7-33-97 cultivars, revealing the similar or differential changes of gene expressions between these two cultivars. This study has improved our overall understanding of the molecular mechanisms of rubber tree resistance to powdery mildew.
白粉病是由橡胶树粉孢（Oidium heveae B. A. Steinmann）引起的橡胶树重要病害。据我们所知，尚未从橡胶树中分离出与白粉病相关的抗性基因。在分子水平上，关于橡胶树如何形成针对这种病原体的防御机制的信息很少。我们通过差异显示分析研究了抗性品种RRIC52的橡胶树mRNA转录本。在接种橡胶树粉孢孢子后的0至120小时内收集叶片，以鉴定不同感染阶段病原体调控的基因。我们鉴定出78个在植物 - 病原体相互作用期间差异表达的橡胶树基因。对这78个EST进行的BLAST分析将它们分为七个功能组：细胞壁和膜途径、转录因子和调节蛋白、转运蛋白、信号转导、植保素生物合成、其他代谢功能以及未知功能。其中八个基因的表达通过qRT-PCR在RRIC52和部分易感的热研7-33-97品种中得到验证，揭示了这两个品种之间基因表达的相似或差异变化。本研究增进了我们对橡胶树抗白粉病分子机制的整体理解。
