阿魏花和叶提取物在体外抑制血管生成。

Ferula gummosa Boiss flower and leaf extracts inhibit angiogenesis in vitro.

作者信息

Mirzaaghaei S, Akrami H, Asadi M H, Mahdiuni H

机构信息

Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.

出版信息

Indian J Cancer. 2014 Oct-Dec;51(4):615-20. doi: 10.4103/0019-509X.175323.

DOI:10.4103/0019-509X.175323

PMID:26842214

Abstract

BACKGROUND

Angiogenesis is a vital process in development as well as in tumor metastasis. Therefore, inhibition of tumor angiogenesis may be an approach for cancer therapy. In this study, we evaluated the effect of Ferula gummosa Boiss flower and leaf extracts on angiogenesis.

MATERIALS AND METHODS

Cell growth and cytotoxic effects of different concentrations (0-70 μg/mL) of F. gummosa Boiss flower and leaf extracts were evaluated on the growth of human umbilical vein endothelial cells (HUVECs) using Neutral Red assay. Then, wound healing, in vitro angiogenesis assay and quantitative VEGF gene expression analysis were conducted with the noncytotoxic concentrations of the ethanol extract.

RESULTS

Our results indicated that observed HUVECs viability was higher than 60% for both extracts after 24 hours treatment at concentration of 30 μg/mL or lower, whereas cytotoxic effects were observed at higher concentrations or after 48 hours treatment. F. gummosa Boiss flower and leaf extracts inhibited migration and angiogenesis capacity in a concentration-dependent manner (10-30 μg/mL), and down regulated VEGF transcription (20 μg/mL for flower extract and 30 μg/mL for leaf extract).

CONCLUSIONS

Our findings revealed that F. gummosa Boiss flower and leaf extracts may contain antiangiogenic compounds, which could be used in preparation of new therapeutic agents for inhibition of tumor angiogenesis. To the best of our knowledge, this is the first report of antiangiogenic effects of F. gummosa Boiss flower and leaf extracts and more studies are needed to identify the effective components of the extracts.

摘要

背景

血管生成是发育以及肿瘤转移过程中的一个重要过程。因此，抑制肿瘤血管生成可能是一种癌症治疗方法。在本研究中，我们评估了阿魏花和叶提取物对血管生成的影响。

材料与方法

使用中性红试验评估不同浓度（0 - 70μg/mL）的阿魏花和叶提取物对人脐静脉内皮细胞（HUVECs）生长的细胞生长和细胞毒性作用。然后，用乙醇提取物的无细胞毒性浓度进行伤口愈合、体外血管生成试验和VEGF基因表达定量分析。

结果

我们的结果表明，在30μg/mL或更低浓度下处理24小时后，两种提取物观察到的HUVECs活力均高于60%，而在更高浓度或处理48小时后观察到细胞毒性作用。阿魏花和叶提取物以浓度依赖性方式（10 - 30μg/mL）抑制迁移和血管生成能力，并下调VEGF转录（花提取物为20μg/mL，叶提取物为30μg/mL）。