阿魏属 Gumosa 博伊斯提取物对人乳腺癌细胞系的细胞毒性和凋亡作用。
Cytotoxic and apoptotic effects of Ferula gummosa Boiss: extract on human breast adenocarcinoma cell line.
机构信息
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Mol Biol Rep. 2024 Apr 29;51(1):592. doi: 10.1007/s11033-024-09364-1.
PURPOSE
Ferula gummosa Boiss. is a well-known and valuable medicinal plant in Iran. Research has shown that this plant has several pharmacological properties, including anti-bacterial, anti-cancer and etc. In the present study, we investigated the cytotoxic properties of F. gummosa Boiss. extract in MCF-7 breast adenocarcinoma cells.
METHODS
The cytotoxicity and pro-apoptotic properties of the extract were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and propidium iodide (PI) stained cells, respectively. Apoptosis and necrosis were evaluated by annexin V-PI staining. The levels of reactive oxygen species (ROS),malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) was determined to evaluate oxidative stress. The cell migration and the gene expression were assessed by scratch assay and quantitative real-time polymerase chain reaction (q-RT-PCR), respectively.
RESULTS
The extract of F. gummosa decreased the viability and cell cycle progression of MCF-7 cells by inducing apoptosis and necrosis, increasing ROS and MDA levels, and decreasing GSH levels and SOD activity. It also lowered the cells' migration capability by enhancing p53 mRNA levels and reducing MMP-9 mRNA expression.
CONCLUSION
F. gummosa exhibited pro-apoptotic, anti-proliferative, and anti-metastatic effects on MCF-7 cells. It is therefore recommended that detailed future research be done on different parts of the plant or its secondary metabolites to find anti-cancer lead compounds.
目的
阿魏是伊朗一种广为人知且具有重要价值的药用植物。研究表明，该植物具有多种药理学特性，包括抗菌、抗癌等。本研究旨在探讨阿魏提取物对 MCF-7 乳腺癌细胞的细胞毒性作用。
方法
通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验和碘化丙啶（PI）染色细胞分别评估提取物的细胞毒性和促凋亡作用。通过 Annexin V-PI 染色评估细胞凋亡和坏死。通过测定活性氧（ROS）、丙二醛（MDA）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）的水平来评估氧化应激。通过划痕试验和实时定量聚合酶链反应（q-RT-PCR）分别评估细胞迁移和基因表达。
结果
阿魏提取物通过诱导细胞凋亡和坏死，增加 ROS 和 MDA 水平，降低 GSH 水平和 SOD 活性，降低 MCF-7 细胞的活力和细胞周期进程。它还通过增加 p53 mRNA 水平和降低 MMP-9 mRNA 表达来降低细胞迁移能力。
结论
阿魏对 MCF-7 细胞具有促凋亡、抗增殖和抗转移作用。因此，建议对该植物的不同部位或其次生代谢产物进行详细的进一步研究，以寻找抗癌的先导化合物。
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