Li Xuezhou, Ding Jianxun, Zhang Zhengzheng, Yang Modi, Yu Jiakuo, Wang Jincheng, Chang Fei, Chen Xuesi
Department of Orthopaedics, The Second Hospital of Jilin University , Changchun 130041, People's Republic of China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, People's Republic of China.
ACS Appl Mater Interfaces. 2016 Mar 2;8(8):5148-59. doi: 10.1021/acsami.5b12212. Epub 2016 Feb 16.
Recently, cartilage tissue engineering (CTE) attracts increasing attention in cartilage defect repair. In this work, kartogenin (KGN), an emerging chondroinductive nonprotein small molecule, was incorporated into a thermogel of poly(L-lactide-co-glycolide)-poly(ethylene glycol)-poly(L-lactide-co-glycolide) (PLGA-PEG-PLGA) to fabricate an appropriate microenvironment of bone marrow mesenchymal stem cells (BMSCs) for effective cartilage regeneration. More integrative and smoother repaired articular surface, more abundant characteristic glycosaminoglycans (GAGs) and collagen II (COL II), and less degeneration of normal cartilage were obtained in the KGN and BMSCs coloaded thermogel group in vivo. In conclusion, the KGN-loaded PLGA-PEG-PLGA thermogel can be utilized as an alternative support for BMSCs to regenerate damaged cartilage in vivo.
近年来，软骨组织工程（CTE）在软骨缺损修复方面越来越受到关注。在本研究中，将一种新兴的具有软骨诱导作用的非蛋白质小分子卡托金（KGN）掺入聚（L-丙交酯-共-乙交酯）-聚（乙二醇）-聚（L-丙交酯-共-乙交酯）（PLGA-PEG-PLGA）热凝胶中，以构建适合骨髓间充质干细胞（BMSCs）的微环境，实现有效的软骨再生。在体内，KGN与BMSCs共负载热凝胶组获得了更完整、更光滑的修复关节表面，更丰富的特征性糖胺聚糖（GAGs）和胶原蛋白II（COL II），以及更少的正常软骨退变。总之，负载KGN的PLGA-PEG-PLGA热凝胶可作为BMSCs在体内再生受损软骨的替代支架。
