Buckley K, Atkins C G, Chen D, Schulze H G, Devine D V, Blades M W, Turner R F B
Michael Smith Laboratories, The University of British Columbia, 2185 East Mall, Vancouver, BC, Canada V6 T 1Z4.
Department of Pathology and Laboratory Medicine, The University of British Columbia, 2211 Wesbrook Mall, Vancouver, BC, Canada V6 T 2B5 and Centre for Blood Research, The University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6 T 1Z3.
Analyst. 2016 Mar 7;141(5):1678-85. doi: 10.1039/c5an02461g.
After being separated from (donated) whole blood, red blood cells are suspended in specially formulated additive solutions and stored (at 4 °C) in polyvinyl chloride (PVC) blood-bags until they are needed for transfusion. With time, the prepared red cell concentrate (RCC) is known to undergo biochemical changes that lower effectiveness of the transfusion, and thus regulations are in place that limit the storage period to 42 days. At present, RCC is not subjected to analytical testing prior to transfusion. In this study, we use Spatially Offset Raman Spectroscopy (SORS) to probe, non-invasively, the biochemistry of RCC inside sealed blood-bags. The retrieved spectra compare well with conventional Raman spectra (of sampled aliquots) and are dominated by features associated with hemoglobin. In addition to the analytical demonstration that SORS can be used to retrieve RCC spectra from standard clinical blood-bags without breaking the sterility of the system, the data reveal interesting detail about the oxygenation-state of the stored cells themselves, namely that some blood-bags unexpectedly contain measurable amounts of deoxygenated hemoglobin after weeks of storage. The demonstration that chemical information can be obtained non-invasively using spectroscopy will enable new studies of RCC degeneration, and points the way to a Raman-based instrument for quality-control in a blood-bank or hospital setting.
从（捐献的）全血中分离出来后，红细胞被悬浮在特制的添加剂溶液中，并（在4°C下）储存在聚氯乙烯（PVC）血袋中，直至输血需要时使用。随着时间的推移，已知制备好的红细胞浓缩液（RCC）会发生生化变化，从而降低输血效果，因此制定了相关规定，将储存期限制在42天。目前，RCC在输血前不进行分析检测。在本研究中，我们使用空间偏移拉曼光谱（SORS）对密封血袋内的RCC生化特性进行非侵入式探测。获取的光谱与（取样等分试样的）传统拉曼光谱匹配良好，且主要由与血红蛋白相关的特征主导。除了通过分析证明SORS可用于从标准临床血袋中获取RCC光谱而不破坏系统无菌性外，数据还揭示了关于储存细胞自身氧合状态的有趣细节，即一些血袋在储存数周后意外地含有可测量量的脱氧血红蛋白。利用光谱学非侵入式获取化学信息的证明将有助于开展关于RCC退化的新研究，并为血库或医院环境中的质量控制提供一种基于拉曼的仪器指明了方向。
