Lukose Jijo, N Mithun, Mohan Ganesh, Shastry Shamee, Chidangil Santhosh
Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education Manipal Karnataka India-576104
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Academy of Higher Education Manipal Karnataka India-576104.
RSC Adv. 2019 Mar 11;9(14):7878-7884. doi: 10.1039/c8ra10061f. eCollection 2019 Mar 6.
The use of normal saline for washing red blood cells and treating critically ill patients is a regular medical practice in hospital settings. An optical tweezer in combination with Raman spectroscopy is an analytical tool employed for the investigation of single cell dynamics, thus providing molecular fingerprint of the cell by optically trapping the cell at a laser focus. In this study, the impact of normal saline on individual human red blood cell was compared with that of blood plasma using Raman tweezers spectroscopy. Major spectral variations in the marker frequencies at 1209 cm, 1222 cm, 1544 cm, and 1561 cm of the Raman spectrum of the treated cells imply that the transition of hemoglobin to the deoxygenated state occurs when 0.9% normal saline is used. This may result in serious implications in blood transfusion. The results obtained from the principal component analysis also displayed clear differentiation among the red blood cells diluted in normal saline and those diluted in plasma. In future studies, efforts will be made to correlate the deoxygenation status of red blood cells with various human disorders.
在医院环境中，使用生理盐水冲洗红细胞并治疗重症患者是常规医疗操作。光学镊子与拉曼光谱相结合是一种用于研究单细胞动力学的分析工具，通过在激光焦点处光学捕获细胞来提供细胞的分子指纹。在本研究中，使用拉曼镊子光谱比较了生理盐水和血浆对单个人类红细胞的影响。处理后细胞拉曼光谱在1209 cm、1222 cm、1544 cm和1561 cm处标记频率的主要光谱变化表明，使用0.9%生理盐水时血红蛋白会转变为脱氧状态。这可能在输血中产生严重影响。主成分分析得到的结果也显示，用生理盐水稀释的红细胞和用血浆稀释的红细胞之间有明显差异。在未来的研究中，将努力把红细胞的脱氧状态与各种人类疾病联系起来。
