Antonov Dimitar, Schliemann Sibylle, Elsner Peter
Curr Probl Dermatol. 2016;49:61-70. doi: 10.1159/000441546. Epub 2016 Feb 4.
Due to the ease of skin accessibility, a large variety of invasive and noninvasive in vitro and in vivo methods have been developed to study barrier function. The measurement of the transepidermal water loss (TEWL) is most widely used in clinical studies. The different methods of determining TEWL, as well as skin hydration, skin pH, tape stripping and other modern less widely used methods to assess skin barrier function, are reviewed, including Raman spectroscopy and imaging methods such as optical coherence tomography and laser scanning microscopy. The modern imaging methods are important developments in the last decades which, however, determine the structure and, hence, cannot replace the measurement of TEWL in questions related to function.
由于皮肤易于接触，人们开发了各种各样的侵入性和非侵入性体外和体内方法来研究屏障功能。经表皮水分流失（TEWL）的测量在临床研究中应用最为广泛。本文综述了测定TEWL的不同方法，以及皮肤水合作用、皮肤pH值、胶带剥离法和其他评估皮肤屏障功能但使用不太广泛的现代方法，包括拉曼光谱法和成像方法，如光学相干断层扫描和激光扫描显微镜。现代成像方法是过去几十年中的重要进展，然而，它们只能确定结构，因此在与功能相关的问题上无法取代TEWL的测量。
