Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090, Vienna, Austria.
Skin Res Technol. 2013 Feb;19(1):e259-72. doi: 10.1111/j.1600-0846.2012.00637.x. Epub 2012 Jun 7.
Despite the frequent use of porcine ear skin for tape-stripping experiments, the peculiarities of this skin type have not been characterised in detail yet. Thus, different techniques were employed to investigate the skin surface structure of porcine ear skin and the changes in barrier function during in vitro tape stripping. To this end, the potential of capacitance-based skin hydration imaging as a means of skin quality control was investigated for the first time.
The porcine ear model was characterised before and during tape stripping using transepidermal water loss (TEWL) measurements, capacitance-based sensor imaging, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and optical light microscopy.
The capacitance-based sensor was found to deliver precise information about the quality of the employed skin sites before and during tape stripping. The removal of stratum corneum proteins was highly reproducible even for different porcine ear types. The mean greyscale values showed an excellent linear correlation to the corresponding TEWL values and the respective penetration depth. Optical light microscopy confirmed the presence of canyons on the surface of porcine ear skin.
The results suggest that the capacitance-based sensor is a suitable tool for skin integrity testing of porcine ear skin in vitro and for monitoring changes in skin barrier function.
尽管猪耳皮肤经常用于胶带剥离实验，但这种皮肤类型的特点尚未详细描述。因此，采用了不同的技术来研究猪耳皮肤的表皮结构和体外胶带剥离过程中屏障功能的变化。为此，首次研究了基于电容的皮肤水合成像作为皮肤质量控制手段的潜力。
在进行胶带剥离之前和期间，使用经表皮水分流失（TEWL）测量、基于电容的传感器成像、衰减全反射傅里叶变换红外光谱（ATR-FTIR）和光学显微镜对猪耳模型进行了特征描述。
发现基于电容的传感器能够在胶带剥离之前和期间提供有关所使用皮肤部位质量的精确信息。即使对于不同的猪耳类型，角质层蛋白的去除也具有高度的重现性。平均灰度值与相应的 TEWL 值和各自的穿透深度之间存在极好的线性相关性。光学显微镜证实了猪耳皮肤表面存在峡谷。
结果表明，基于电容的传感器是体外猪耳皮肤完整性测试和监测皮肤屏障功能变化的合适工具。
