Halfter H, Müller U, Winnacker E L, Gallwitz D
Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG.
EMBO J. 1989 Oct;8(10):3029-37. doi: 10.1002/j.1460-2075.1989.tb08453.x.
We have identified a protein, BAF1, which has two oppositely oriented, partially overlapping binding sites within a symmetrical sequence located midway between and upstream of the divergently transcribed YPT1 and TUB2 genes of the yeast Saccharomyces cerevisiae. The 120 kd BAF1 protein was purified to near homogeneity and used to delineate the two binding sites and to identify apparent protein contact sites by the missing contact technique, methylation interference and by site-directed mutagenesis. The BAF1-recognition sequence contains a conserved TCN7ACG element recently identified at autonomously replicating sequences (ARS) and in the 5' and 3' flanking region of other yeast genes. The symmetrical sequence of the YPT1/TUB2 intergene region seems not to be involved in DNA replication but activates transcription in an orientation-independent fashion.
我们已经鉴定出一种蛋白质BAF1，它在酿酒酵母中两个反向转录的YPT1和TUB2基因之间且位于其上游的对称序列内，有两个方向相反、部分重叠的结合位点。120kd的BAF1蛋白被纯化至近乎同质，并用于描绘这两个结合位点，以及通过缺失接触技术、甲基化干扰和定点诱变来鉴定明显的蛋白质接触位点。BAF1识别序列包含一个最近在自主复制序列（ARS）以及其他酵母基因的5'和3'侧翼区域中鉴定出的保守的TCN7ACG元件。YPT1/TUB2基因间区域的对称序列似乎不参与DNA复制，而是以方向独立的方式激活转录。
