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大肠杆菌RNA聚合酶与噬菌体T7早期启动子之间的相互作用。

Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.

作者信息

Siebenlist U, Gilbert W

出版信息

Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6. doi: 10.1073/pnas.77.1.122.

DOI:10.1073/pnas.77.1.122
PMID:6987648
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348220/
Abstract

Specific contacts between the Escherichia coli RNA polymerase (nucleosidetriphosphate:RNA nucleotidyl-transferase, EC2.7.7.6) and the phosphates and purine bases of the A3 promoter of phage T7 cluster into three regions located approximately 10, 16, and 35 base pairs before RNA initiation site. Two of these contain nucleotide sequences that are fairly conserved among many promoters, known as the "Pribnow box" and "-35 region" homologies; the third, just upstream from the Pribnow box, is not conserved. The polymerase binds preferentially to the coding strand and for the most part touches only one face of the DNA helix.

摘要

大肠杆菌RNA聚合酶（核苷三磷酸：RNA核苷酸转移酶，EC2.7.7.6）与噬菌体T7 A3启动子的磷酸基团和嘌呤碱基之间的特异性接触结合接触到位于RNA起始位点之前约10、16和35个碱基对处的三个区域。其中两个区域包含在许多启动子中相当保守的核苷酸序列，即所谓的“Pribnow框”和“-35区域”同源序列；第三个区域就在Pribnow框的上游，不保守。聚合酶优先与编码链结合，并且在大多数情况下仅接触DNA螺旋的一个面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14c/348220/77ddc877177b/pnas00664-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14c/348220/b2f7d01c7f70/pnas00664-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14c/348220/77ddc877177b/pnas00664-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14c/348220/b2f7d01c7f70/pnas00664-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14c/348220/77ddc877177b/pnas00664-0162-a.jpg

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The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli.适用于大肠杆菌中低背景的多功能克隆的质粒载体 pBS2ndd 和 pBS3ndd。
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