Becker D, Meier C B, Herlyn M
Department of Tumor Biology, M.D. Anderson Cancer Center, Houston, TX 77030.
EMBO J. 1989 Dec 1;8(12):3685-91. doi: 10.1002/j.1460-2075.1989.tb08543.x.
Human malignant melanomas, unlike normal melanocytes, can proliferate in the absence of exogenous basic fibroblast growth factor (bFGF). Exposure of primary melanomas in the vertical growth phase and metastatic melanomas to antisense oligodeoxynucleotides targeted against three different sites of human bFGF mRNA inhibited cell proliferation and colony formation in soft-agar. In contrast, exposure of human bFGF sense or antisense oligonucleotides complementary to human beta-nerve growth factor or insulin-like growth factor I mRNA had no such effects. These experiments indicate that activation of the bFGF gene may play an important role in the progression from melanocytic precursor lesions to malignant melanoma.
与正常黑素细胞不同，人类恶性黑色素瘤在没有外源性碱性成纤维细胞生长因子（bFGF）的情况下也能增殖。将处于垂直生长期的原发性黑色素瘤和转移性黑色素瘤暴露于针对人类bFGF mRNA三个不同位点的反义寡脱氧核苷酸中，可抑制软琼脂中的细胞增殖和集落形成。相比之下，与人类β-神经生长因子或胰岛素样生长因子I mRNA互补的人类bFGF正义或反义寡核苷酸则没有这种作用。这些实验表明，bFGF基因的激活可能在从黑素细胞前体病变发展为恶性黑色素瘤的过程中起重要作用。
