Bell G I, Gerhard D S, Fong N M, Sanchez-Pescador R, Rall L B
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6450-4. doi: 10.1073/pnas.82.19.6450.
Overlapping recombinant clones that encompass the insulin-like growth factor (IGF) I and II genes have been isolated from a human genomic DNA library. Each gene is present once per haploid genome; the IGF-I gene spans greater than 35 kilobase pairs (kbp) and the IGF-II gene is at least 15 kbp. The exon-intron organization of these genes is similar, each having four exons, which is one more than the related insulin gene. Comparison of the restriction endonuclease cleavage maps of the IGF-II and insulin genes, including their flanking regions and hybridization with an IGF-II cDNA probe, revealed that they are adjacent to one another. The IGF-II and insulin genes have the same polarity and are separated by 12.6 kbp of intergenic DNA that includes a dispersed middle repetitive Alu sequence. The order of the genes is 5'-insulin-IGF-II-3'.
已从人类基因组DNA文库中分离出包含胰岛素样生长因子（IGF）I和II基因的重叠重组克隆。每个基因在单倍体基因组中仅出现一次；IGF-I基因跨度大于35千碱基对（kbp），IGF-II基因至少为15 kbp。这些基因的外显子-内含子组织相似，每个都有四个外显子，比相关的胰岛素基因多一个。对IGF-II和胰岛素基因的限制性内切酶切割图谱进行比较，包括它们的侧翼区域，并与IGF-II cDNA探针杂交，结果表明它们彼此相邻。IGF-II和胰岛素基因具有相同的极性，被12.6 kbp的基因间DNA隔开，该DNA包括一个分散的中间重复Alu序列。基因顺序为5'-胰岛素-IGF-II-3'。
