慢病毒介导的长链非编码RNA GAS5过表达通过介导人黑色素瘤细胞中基质金属蛋白酶2（MMP2）的表达和活性来降低侵袭能力。

Lentiviral-mediated overexpression of long non-coding RNA GAS5 reduces invasion by mediating MMP2 expression and activity in human melanoma cells.

作者信息

Chen Long, Yang Huixin, Xiao Yanbin, Tang Xiaoxia, Li Yuqian, Han Qiaoqiao, Fu Junping, Yang Yuye, Zhu Yuechun

机构信息

Department of Biochemistry and Molecular Biology of Kunming Medical University, Yunnan, P.R. China.

Department of Orthopaedic Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan, P.R. China.

出版信息

Int J Oncol. 2016 Apr;48(4):1509-18. doi: 10.3892/ijo.2016.3377. Epub 2016 Feb 4.

DOI:10.3892/ijo.2016.3377

PMID:26846479

Abstract

The present study evaluated the effects of long non-coding RNA GAS5 on the migration and invasion of melanoma cells. Using the SK-Mel‑110 melanoma cell line, we stably expressed GAS5, visualized the distribution of GAS5 by RNA fluorescence in situ hybridization (FISH) and examined changes in cell migration and invasion with Transwell assays. In GAS5 overexpressed SK-Mel‑110 cells, migrated and invaded cells decreased by 65.3 and 55.6%, respectively. Moreover, the MMP2 protein level, and its activity was downregulated by 67.9 and 15.8%, respectively. Overexpressing lncRNA GAS5 inhibited the migration and invasion ability of melanoma SK-Mel‑110 cells, partially by decreasing the MMP2 expression and its activity. This study is the first to reveal a potential relationship between lncRNA GAS5 and the migration and invasion of melanoma.

摘要

本研究评估了长链非编码RNA GAS5对黑色素瘤细胞迁移和侵袭的影响。我们使用SK-Mel-110黑色素瘤细胞系稳定表达GAS5，通过RNA荧光原位杂交（FISH）观察GAS5的分布，并采用Transwell实验检测细胞迁移和侵袭的变化。在GAS5过表达的SK-Mel-110细胞中，迁移和侵袭的细胞分别减少了65.3%和55.6%。此外，MMP2蛋白水平及其活性分别下调了67.9%和15.8%。过表达lncRNA GAS5抑制了黑色素瘤SK-Mel-110细胞的迁移和侵袭能力，部分原因是降低了MMP2的表达及其活性。本研究首次揭示了lncRNA GAS5与黑色素瘤迁移和侵袭之间的潜在关系。