Tan Qiyan, Liu Changyu, Shen Ying, Huang Tao
Department of Laboratory, Hainan People's Hospital, Haikou, Hainan, China.
Department of Laboratory, Hainan People's Hospital, Haikou, Hainan, China. Email:
Cell J. 2021 Oct;23(5):552-561. doi: 10.22074/cellj.2021.7913. Epub 2021 Oct 30.
There is growing evidence showing that circular RNAs (circRNAs) are crucial regulators in modulating the biological behavior of tumors. This work is aimed to probe the role of in non-small cell lung cancer (NSCLC) and to elucidate its mechanism of action.
In this experimental study, the differentially expressed circRNAs in NSCLC were screened using the GEO database (GSE158695). , and expression levels were determined by quantitative real-time polymerase chain reaction (qRT-PCR) analysis and Western blot. The proliferation, apoptosis, migration, and invasion of NSCLC cells were detected by CCK-8, flow cytometry, and transwell assays. RNA immunoprecipitation (RIP), RNA pull-down, and dual-luciferase reporter gene assays were performed to clarify the association between the and .
was shown to be up-regulated in NSCLC tissues and cell lines. The up-regulation of is closely associated with advanced clinical stage of cancer, lymph node metastasis, and poor prognosis in NSCLC patients. knockdown impeded the proliferation, migration, and invasion of NSCLC cells and enhanced their apoptosis. Mechanistically, was demonstrated to up-regulate MMP2 expression via decoying and to facilitate the malignant biological behaviors of NSCLC cells.
This work reveals that is implicated in NSCLC cell growth and metastasis through the modulation of miR-326/miR-330-5p/MMP2, providing novel insights into the role of circRNAs in NSCLC progression.
越来越多的证据表明，环状RNA（circRNAs）是调节肿瘤生物学行为的关键调控因子。本研究旨在探讨[具体circRNA名称未给出]在非小细胞肺癌（NSCLC）中的作用，并阐明其作用机制。
在本实验研究中，使用GEO数据库（GSE158695）筛选NSCLC中差异表达的circRNAs。通过定量实时聚合酶链反应（qRT-PCR）分析和蛋白质免疫印迹法测定[具体circRNA名称未给出]、[其他相关分子名称未给出]和[另一相关分子名称未给出]的表达水平。采用CCK-8、流式细胞术和Transwell实验检测NSCLC细胞的增殖、凋亡、迁移和侵袭能力。进行RNA免疫沉淀（RIP）、RNA下拉和双荧光素酶报告基因实验，以阐明[具体circRNA名称未给出]与[其他相关分子名称未给出]之间的关联。
[具体circRNA名称未给出]在NSCLC组织和细胞系中呈上调表达。[具体circRNA名称未给出]的上调与癌症晚期临床分期、淋巴结转移以及NSCLC患者的不良预后密切相关。敲低[具体circRNA名称未给出]可抑制NSCLC细胞的增殖、迁移和侵袭，并增强其凋亡。机制上，[具体circRNA名称未给出]通过诱饵吸附[其他相关分子名称未给出]上调MMP2表达，促进NSCLC细胞的恶性生物学行为。
本研究揭示[具体circRNA名称未给出]通过调节miR-326/miR-330-5p/MMP2参与NSCLC细胞的生长和转移，为circRNAs在NSCLC进展中的作用提供了新的见解。
